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    Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers.

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    Authors
    Young, A R
    Potten, Christopher S
    Nikaido, O
    Parsons, P G
    Boenders, Johathan
    Ramsden, Jonathan M
    Chadwick, Caroline A
    Affiliation
    Department of Photobiology, St John's Institute for Dermatology, St Thomas Hospital, London, UK.
    Issue Date
    1998-12
    
    Metadata
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    Abstract
    Epidemiology shows a relationship between solar exposure and all types of skin cancer. Understanding the mechanisms of skin cancer requires knowledge of the photomolecular events that occur within the relevant epidermal cell types in vivo. Studies to date have focused on UVR-induced DNA lesions in keratinocytes, the majority epidermal cell population which gives rise to most skin cancers. Malignant melanoma, arising from melanocytes (5%-10% of epidermal cells), accounts for most skin cancer deaths. We report on new techniques to detect DNA photolesions in human epidermal melanocytes in situ. Previously nonexposed buttock skin of volunteers of skin types I/II was exposed to clinically relevant doses of narrow bandwidth UVB (300 nm) and UVA (320 nm, 340 nm, 360 nm) radiation. Biopsies were taken immediately afterwards and processed for routine histology. Microscope sections were prepared and double-stained with fluorescent-tagged monoclonal antibodies for thymine dimers and melanocytes. UVR dose-response curves for dimer levels within melanocyte nuclei were determined by image analysis and compared with dimer levels in adjacent basal cell keratinocytes. Our data show that UVB and UVA readily induce thymine dimers in melanocytes at levels that are comparable with those found in adjacent keratinocytes. This new technique will enable melanocyte specific studies, such as DNA repair kinetics, to be done in vivo.
    Citation
    Human melanocytes and keratinocytes exposed to UVB or UVA in vivo show comparable levels of thymine dimers. 1998, 111 (6):936-40 J. Invest. Dermatol.
    Journal
    The Journal of Investigative Dermatology
    URI
    http://hdl.handle.net/10541/93090
    DOI
    10.1046/j.1523-1747.1998.00435.x
    PubMed ID
    9856799
    Type
    Article
    Language
    en
    ISSN
    0022-202X
    ae974a485f413a2113503eed53cd6c53
    10.1046/j.1523-1747.1998.00435.x
    Scopus Count
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    All Paterson Institute for Cancer Research

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