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dc.contributor.authorYoung, A R
dc.contributor.authorChadwick, Caroline A
dc.contributor.authorHarrison, G I
dc.contributor.authorNikaido, O
dc.contributor.authorRamsden, Jonathan M
dc.contributor.authorPotten, Christopher S
dc.date.accessioned2010-02-25T16:24:49Z
dc.date.available2010-02-25T16:24:49Z
dc.date.issued1998-12
dc.identifier.citationThe similarity of action spectra for thymine dimers in human epidermis and erythema suggests that DNA is the chromophore for erythema. 1998, 111 (6):982-8 J. Invest. Dermatol.en
dc.identifier.issn0022-202X
dc.identifier.pmid9856805
dc.identifier.doi10.1046/j.1523-1747.1998.00436.x
dc.identifier.urihttp://hdl.handle.net/10541/93089
dc.description.abstractThe location of DNA photodamage within the epidermis is crucial as basal layer cells are the most likely to have carcinogenic potential. We have determined the action spectra for DNA photodamage in different human epidermal layers in situ. Previously unexposed buttock skin was irradiated with 0.5, 1, 2, and 3 minimal erythema doses of monochromatic UVR at 280, 290, 300, 310, 320, 340, and 360 nm. Punch biopsies were taken immediately after exposure and paraffin sections were prepared for immunoperoxidase staining with a monoclonal antibody against thymine dimers that were quantitated by image analysis. Dimers were measured at two basal layer regions, the mid and the upper living epidermis. The slopes of dose-response curves were used to generate four action spectra, all of which had maxima at 300 nm. Dimer action spectra between 300 and 360 nm were independent of epidermal layer, indicating comparable epidermal transmission at these wavelengths. Furthermore, we observed 300 nm-induced dimers in dermal nuclei; however, there was a marked effect of epidermal layer between 280 and 300 nm, showing relatively poor transmission of 280 and 290 nm to the basal layer. These data indicate that solar UVB (approximately 295-320 nm) is more damaging to basal cells than predicted from transmission data obtained from human epidermis ex vivo. The epidermal dimer action spectra were compared with erythema action spectra determined from the same volunteers and ultraviolet radiation sources. Overall, these spectral comparisons suggest that DNA is a major chromophore for erythema in the 280-340 nm region.
dc.language.isoenen
dc.subject.meshAdult
dc.subject.meshChromogenic Compounds
dc.subject.meshDNA Damage
dc.subject.meshEpidermis
dc.subject.meshErythema
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshOptical Rotatory Dispersion
dc.subject.meshPhotochemistry
dc.subject.meshPyrimidine Dimers
dc.subject.meshSpectrophotometry, Ultraviolet
dc.titleThe similarity of action spectra for thymine dimers in human epidermis and erythema suggests that DNA is the chromophore for erythema.en
dc.typeArticleen
dc.contributor.departmentDepartment of Photobiology, St John's Institute of Dermatology, Guy's, King's and St Thomas' School of Medicine, University of London, UK.en
dc.identifier.journalThe Journal of Investigative Dermatologyen
html.description.abstractThe location of DNA photodamage within the epidermis is crucial as basal layer cells are the most likely to have carcinogenic potential. We have determined the action spectra for DNA photodamage in different human epidermal layers in situ. Previously unexposed buttock skin was irradiated with 0.5, 1, 2, and 3 minimal erythema doses of monochromatic UVR at 280, 290, 300, 310, 320, 340, and 360 nm. Punch biopsies were taken immediately after exposure and paraffin sections were prepared for immunoperoxidase staining with a monoclonal antibody against thymine dimers that were quantitated by image analysis. Dimers were measured at two basal layer regions, the mid and the upper living epidermis. The slopes of dose-response curves were used to generate four action spectra, all of which had maxima at 300 nm. Dimer action spectra between 300 and 360 nm were independent of epidermal layer, indicating comparable epidermal transmission at these wavelengths. Furthermore, we observed 300 nm-induced dimers in dermal nuclei; however, there was a marked effect of epidermal layer between 280 and 300 nm, showing relatively poor transmission of 280 and 290 nm to the basal layer. These data indicate that solar UVB (approximately 295-320 nm) is more damaging to basal cells than predicted from transmission data obtained from human epidermis ex vivo. The epidermal dimer action spectra were compared with erythema action spectra determined from the same volunteers and ultraviolet radiation sources. Overall, these spectral comparisons suggest that DNA is a major chromophore for erythema in the 280-340 nm region.


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