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    Structural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line.

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    Authors
    Brickman, Y G
    Nurcombe, V
    Ford, M D
    Gallagher, John T
    Bartlett, P F
    Turnbull, Jeremy E
    Affiliation
    Department of Anatomy and Cell Biology, University of Melbourne, Victoria, Australia.
    Issue Date
    1998-05
    
    Metadata
    Show full item record
    Abstract
    Heparan sulfate (HS) glycosaminoglycans are essential modulators of fibroblast growth factor (FGF) activity both in vivo and in vitro, and appear to act by cross-linking particular forms of FGF to appropriate FGF receptors. We have recently isolated and characterized two separate HS pools derived from immortalized embryonic day 10 mouse neuroepithelial 2.3D cells: one from cells in log growth phase, which greatly potentiates the activity of FGF-2, and the other from cells undergoing contact-inhibition and differentiation, which preferentially activates FGF-1. These two pools of HS have very similar functional activities to those species isolated from primary neuroepithelial cells at corresponding stages of active proliferation or differentiation. We present here a structural comparison between these cell line HS species to establish the nature of the changes that occur in the biosynthesis of HS. A combination of chemical and enzymatic cleavage, low pressure chromatography and strong anion-exchange HPLC were used to generate full chain models of each species. Overall, the HS pools synthesized in the dividing cell line pools possessed less complex sulfation than those derived from more differentiated, growth arrested cells.
    Citation
    Structural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line. 1998, 8 (5):463-71 Glycobiology
    Journal
    Glycobiology
    URI
    http://hdl.handle.net/10541/93005
    PubMed ID
    9597544
    Type
    Article
    Language
    en
    ISSN
    0959-6658
    Collections
    All Paterson Institute for Cancer Research

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