CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
dc.contributor.author | De Wynter, Erika A | |
dc.contributor.author | Buck, D | |
dc.contributor.author | Hart, Claire A | |
dc.contributor.author | Heywood, R | |
dc.contributor.author | Coutinho, Lucia H | |
dc.contributor.author | Clayton, Alison J | |
dc.contributor.author | Rafferty, Joseph A | |
dc.contributor.author | Burt, Deborah J | |
dc.contributor.author | Guenechea, G | |
dc.contributor.author | Bueren, J A | |
dc.contributor.author | Gagen, D | |
dc.contributor.author | Fairbairn, Leslie J | |
dc.contributor.author | Lord, Brian I | |
dc.contributor.author | Testa, Nydia G | |
dc.date.accessioned | 2010-02-25T11:38:03Z | |
dc.date.available | 2010-02-25T11:38:03Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. 1998, 16 (6):387-96 Stem Cells | en |
dc.identifier.issn | 1066-5099 | |
dc.identifier.pmid | 9831864 | |
dc.identifier.doi | 10.1002/stem.160387 | |
dc.identifier.uri | http://hdl.handle.net/10541/93003 | |
dc.description.abstract | The AC133 antigen is a novel antigen selectively expressed on a subset of CD34+ cells in human fetal liver, bone marrow, and blood as demonstrated by flow cytometric analyses. In this study, we have further assessed the expression of AC133 on CD34+ cells in hemopoietic samples and found that there was a highly significant difference between normal bone marrow and cord blood versus aphereses (p <0.0001) but not between bone marrow and cord blood. Most of the clonogenic cells (67%) were contained in the CD34+AC133+ fraction. Compared with cultures of the CD34+AC133- cells, generation of progenitor cells in long-term culture on bone marrow stroma was consistently 10- to 100-fold higher in cultures initiated with CD34+AC133+ cells and was maintained for the 8-10 weeks of culture. Only the CD34+AC133+ cells were capable of repopulating NOD/SCID mice. Human cells were detectable as early as day 20, with increased levels (67%) apparent 40 days post-transplantation. Five thousand CD34+AC133+ cells engrafted about 20% of the mice, while no engraftment was observed in animals transplanted with up to 1.2 x 10(5) CD34+AC133- cells. The CD34+AC133+ population was also enriched (seven-fold) in dendritic cell precursors, and the dendritic cells generated were functionally active in a mixed lymphocyte reaction assay. AC133+ cells should be useful in the study of cellular and molecular mechanisms regulating primitive hemopoietic cells. | |
dc.language.iso | en | en |
dc.subject | Foetal Blood | en |
dc.subject | Haematopoiesis | en |
dc.subject | Haematopoietic Stem Cells | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Antigens, CD | |
dc.subject.mesh | Antigens, CD34 | |
dc.subject.mesh | Cell Culture Techniques | |
dc.subject.mesh | Dendritic Cells | |
dc.subject.mesh | Fetal Blood | |
dc.subject.mesh | Flow Cytometry | |
dc.subject.mesh | Glycoproteins | |
dc.subject.mesh | Hematopoiesis | |
dc.subject.mesh | Hematopoietic Stem Cells | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Mice, Inbred NOD | |
dc.subject.mesh | Mice, SCID | |
dc.subject.mesh | Peptides | |
dc.subject.mesh | Time Factors | |
dc.title | CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. | en |
dc.type | Article | en |
dc.contributor.department | CRC Section of Haemopoietic Cell and Gene Therapeutics, Paterson Institute for Cancer Research, Withington, Manchester, United Kingdom. | en |
dc.identifier.journal | Stem Cells | en |
html.description.abstract | The AC133 antigen is a novel antigen selectively expressed on a subset of CD34+ cells in human fetal liver, bone marrow, and blood as demonstrated by flow cytometric analyses. In this study, we have further assessed the expression of AC133 on CD34+ cells in hemopoietic samples and found that there was a highly significant difference between normal bone marrow and cord blood versus aphereses (p <0.0001) but not between bone marrow and cord blood. Most of the clonogenic cells (67%) were contained in the CD34+AC133+ fraction. Compared with cultures of the CD34+AC133- cells, generation of progenitor cells in long-term culture on bone marrow stroma was consistently 10- to 100-fold higher in cultures initiated with CD34+AC133+ cells and was maintained for the 8-10 weeks of culture. Only the CD34+AC133+ cells were capable of repopulating NOD/SCID mice. Human cells were detectable as early as day 20, with increased levels (67%) apparent 40 days post-transplantation. Five thousand CD34+AC133+ cells engrafted about 20% of the mice, while no engraftment was observed in animals transplanted with up to 1.2 x 10(5) CD34+AC133- cells. The CD34+AC133+ population was also enriched (seven-fold) in dendritic cell precursors, and the dendritic cells generated were functionally active in a mixed lymphocyte reaction assay. AC133+ cells should be useful in the study of cellular and molecular mechanisms regulating primitive hemopoietic cells. |