Altered stem cell regeneration in irradiated intestinal crypts of senescent mice.
AffiliationBiological Gerontology Group, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
MetadataShow full item record
AbstractAgeing is associated with a progressive deterioration in the functions of many organs within the body. In tissue with high cell turnover, the maintenance of the stem cells is of particular importance. Any accumulation of damage in stem cells may affect their function and hence threaten the homeostasis and regenerative capacity of the tissue. The small intestine represents a good model for the study of stem cells because of its spatial and hierarchical organisation. We have examined the effect of age on stem cell regenerative capacity after irradiation, using the microcolony assay. Crypt survival levels, the growth rate of surviving crypts, and the number of cells able to repopulate a crypt have been investigated by irradiating groups of 6-7 month old and 28-30 month old ICRFa male mice. After high doses of irradiation, the surviving crypts in old mice were both smaller and fewer in number than in young mice. The growth rate of surviving crypts was determined by measuring the crypt area and the number of cells/crypt at various times after 14 Gy irradiation. There was a growth delay of between about one half and one day in the older mice. Surprisingly, the number of clonogenic cells per crypt was estimated to be greater in the older mice. These studies indicate important age-related alterations in the capacity to regenerate the crypts after radiation damage.
CitationAltered stem cell regeneration in irradiated intestinal crypts of senescent mice. 1998, 111 ( Pt 16):2297-303 J. Cell. Sci.
JournalJournal of Cell Science
- The effect of proliferative status and clonogen content on the response of mouse jejunal crypts to split-dose irradiation.
- Authors: Thames HD, Ruifrok AC, Mason KA
- Issue date: 1997 Feb
- Enhancement of murine intestinal stem cell survival after irradiation by keratinocyte growth factor.
- Authors: Khan WB, Shui C, Ning S, Knox SJ
- Issue date: 1997 Sep
- Age changes in stem cells of murine small intestinal crypts.
- Authors: Martin K, Kirkwood TB, Potten CS
- Issue date: 1998 Jun 15
- Ageing of murine small intestinal stem cells.
- Authors: Potten CS, Martin K, Kirkwood TB
- Issue date: 2001
- In vivo radioprotective effects of angiogenic growth factors on the small bowel of C3H mice.
- Authors: Okunieff P, Mester M, Wang J, Maddox T, Gong X, Tang D, Coffee M, Ding I
- Issue date: 1998 Aug