AffiliationDepartment of Geriatric Medicine and School of Biological Sciences, University of Manchester, 3.239 Stopford Building, Oxford Road, Manchester, M13 9PT, United Kingdom.
MetadataShow full item record
AbstractCell senescence is seen in many types of differentiated cells but age changes in stem cells have not previously been clearly demonstrated. Changes in stem cells may be of great importance for the ageing process, because any decline with age in the numbers and functional integrity of stem cells can lead to progressive deterioration of function and of proliferative homeostasis in tissues. Stem cells of the murine small intestine provide an excellent model system because these cells occupy a well-defined position near the base of the crypts of Lieberkühn. We examined mice aged between 5 and 32 months and found age-related alterations in the histology of the small intestine and in the apoptotic response of stem cells to low-dose irradiation. Apoptosis in the crypts is concentrated around the stem cell position and can be markedly elevated by exposure to radiation or cytotoxic agents, suggesting that "suicide" of damaged stem cells may be an important system for long-term tissue maintenance. Animals aged 5, 15, 18, and 29 months were exposed to either 1 or 8 Gy gamma irradiation. A twofold increase in the level of apoptosis was seen following 1 Gy gamma irradiation in the 29-month-old animals, compared to the young and middle-age groups. After 8 Gy irradiation the level of apoptosis in all age groups was high and the age effect less pronounced. The data suggest that stem cells do undergo some functional alteration with age.
CitationAge changes in stem cells of murine small intestinal crypts. 1998, 241 (2):316-23 Exp. Cell Res.
JournalExperimental Cell Research
- The effects of repeated doses of keratinocyte growth factor on cell proliferation in the cellular hierarchy of the crypts of the murine small intestine.
- Authors: Potten CS, O'Shea JA, Farrell CL, Rex K, Booth C
- Issue date: 2001 May
- Activation of nuclear factor kappaB In vivo selectively protects the murine small intestine against ionizing radiation-induced damage.
- Authors: Wang Y, Meng A, Lang H, Brown SA, Konopa JL, Kindy MS, Schmiedt RA, Thompson JS, Zhou D
- Issue date: 2004 Sep 1
- [Radiobiology of stem cells in intestinal epithelium. Effect of single and multiple preliminary sublethal irradiation of mice on the dose dependence of the survival of stem cells in small intestine epithelium].
- Authors: Konopliannikova OA
- Issue date: 1988 Jan-Feb
- Brca2 deficiency in the murine small intestine sensitizes to p53-dependent apoptosis and leads to the spontaneous deletion of stem cells.
- Authors: Hay T, Patrick T, Winton D, Sansom OJ, Clarke AR
- Issue date: 2005 May 26
- The clonogen content of murine intestinal crypts: dependence on radiation dose used in its determination.
- Authors: Hendry JH, Roberts SA, Potten CS
- Issue date: 1992 Oct