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dc.contributor.authorPierce, A
dc.contributor.authorWhetton, Anthony D
dc.contributor.authorOwen-Lynch, P J
dc.contributor.authorTavernier, J
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorDexter, T Michael
dc.contributor.authorHeyworth, Clare M
dc.date.accessioned2010-02-24T12:09:33Z
dc.date.available2010-02-24T12:09:33Z
dc.date.issued1998-03
dc.identifier.citationEctopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line. 1998, 111 ( Pt 6):815-23 J. Cell. Sci.en
dc.identifier.issn0021-9533
dc.identifier.pmid9472009
dc.identifier.urihttp://hdl.handle.net/10541/92889
dc.description.abstractThe interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific alpha subunit and a common ssc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor alpha subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this alpha subunit. The ectopic expression of the IL-5 receptor alpha subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor alpha subunit.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshGene Expression Regulation
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterleukin-5
dc.subject.meshMice
dc.subject.meshReceptors, Interleukin
dc.subject.meshReceptors, Interleukin-5
dc.subject.meshTransfection
dc.titleEctopic interleukin-5 receptor expression promotes proliferation without development in a multipotent hematopoietic cell line.en
dc.typeArticleen
dc.contributor.departmentLeukemia Research Fund, Cellular Development Unit, UMIST, Manchester M60 1QD, UK.en
dc.identifier.journalJournal of Cell Scienceen
html.description.abstractThe interleukin-5 (IL-5) receptor is a heterodimer that consists of an IL-5 specific alpha subunit and a common ssc chain that is shared with the receptors for granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-3 (IL-3). In contrast to IL-5, which acts mainly as an eosinophil lineage specific factor in vivo, IL-3 and GM-CSF stimulate the survival, proliferation and development of various hematopoietic cell lineages and also multipotent progenitor cells. IL-5 has little effect on the survival or proliferation of the multipotent stem cell line FDCP-Mix A4 but does promote some eosinophil development. To investigate whether the lineage specificity of IL-5 is due to the restricted expression of the IL-5 receptor alpha subunit we transfected the FDCP-Mix A4 cells with a retroviral vector containing this alpha subunit. The ectopic expression of the IL-5 receptor alpha subunit in the FDCP-Mix cells did not increase the observed eosinophilic development but did stimulate survival and proliferation of the transfected cells when IL-5 was added. IL-5 thus acts like IL-3 in these cells, promoting proliferation and survival. The results suggest that IL-5, whilst having a capacity to promote proliferation, does not influence eosinophilic lineage commitment in these multipotent cells. The results further argue that the observed lineage specificity of IL-5 is probably due to factors in addition to the restricted expression of the IL-5 receptor alpha subunit.


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