Show simple item record

dc.contributor.authorPierce, A
dc.contributor.authorHeyworth, Clare M
dc.contributor.authorNicholls, S E
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorDexter, T Michael
dc.contributor.authorLord, J M
dc.contributor.authorOwen-Lynch, P J
dc.contributor.authorWark, G
dc.contributor.authorWhetton, Anthony D
dc.date.accessioned2010-02-23T13:24:17Z
dc.date.available2010-02-23T13:24:17Z
dc.date.issued1998-03-23
dc.identifier.citationAn activated protein kinase C alpha gives a differentiation signal for hematopoietic progenitor cells and mimicks macrophage colony-stimulating factor-stimulated signaling events. 1998, 140 (6):1511-8 J. Cell Biol.en
dc.identifier.issn0021-9525
dc.identifier.pmid9508782
dc.identifier.urihttp://hdl.handle.net/10541/92761
dc.description.abstractHighly enriched, bipotent, hematopoietic granulocyte macrophage colony-forming cells (GM-CFC) require cytokines for their survival, proliferation, and development. GM-CFC will form neutrophils in the presence of the cytokines stem cell factor and granulocyte colony-stimulating factor, whereas macrophage colony-stimulating factor leads to macrophage formation. Previously, we have shown that the commitment to the macrophage lineage is associated with lipid hydrolysis and translocation of protein kinase C alpha (PKCalpha) to the nucleus. Here we have transfected freshly prepared GM-CFC with a constitutively activated form of PKCalpha, namely PKAC, in which the regulatory domain has been truncated. Greater than 95% of the transfected cells showed over a twofold increase in PKCalpha expression with the protein being located primarily within the nucleus. The expression of PKAC caused macrophage development even in the presence of stimuli that normally promote only neutrophilic development. Thus, M-CSF-stimulated translocation of PKCalpha to the nucleus is a signal associated with macrophage development in primary mammalian hematopoietic progenitor cells, and this signal can be mimicked by ectopic PKAC, which is also expressed in the nucleus.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshCulture Media
dc.subject.meshEnzyme Activation
dc.subject.meshFlow Cytometry
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterleukin-3
dc.subject.meshIsoenzymes
dc.subject.meshMice
dc.subject.meshMicroscopy, Confocal
dc.subject.meshProtein Kinase C
dc.subject.meshProtein Kinase C-alpha
dc.subject.meshSignal Transduction
dc.subject.meshTransfection
dc.titleAn activated protein kinase C alpha gives a differentiation signal for hematopoietic progenitor cells and mimicks macrophage colony-stimulating factor-stimulated signaling events.en
dc.typeArticleen
dc.contributor.departmentLeukaemia Research Fund Cellular Development Unit, University of Manchester Institute of Science and Technology, Manchester, M60 1QD, United Kingdom.en
dc.identifier.journalThe Journal of Cell Biologyen
html.description.abstractHighly enriched, bipotent, hematopoietic granulocyte macrophage colony-forming cells (GM-CFC) require cytokines for their survival, proliferation, and development. GM-CFC will form neutrophils in the presence of the cytokines stem cell factor and granulocyte colony-stimulating factor, whereas macrophage colony-stimulating factor leads to macrophage formation. Previously, we have shown that the commitment to the macrophage lineage is associated with lipid hydrolysis and translocation of protein kinase C alpha (PKCalpha) to the nucleus. Here we have transfected freshly prepared GM-CFC with a constitutively activated form of PKCalpha, namely PKAC, in which the regulatory domain has been truncated. Greater than 95% of the transfected cells showed over a twofold increase in PKCalpha expression with the protein being located primarily within the nucleus. The expression of PKAC caused macrophage development even in the presence of stimuli that normally promote only neutrophilic development. Thus, M-CSF-stimulated translocation of PKCalpha to the nucleus is a signal associated with macrophage development in primary mammalian hematopoietic progenitor cells, and this signal can be mimicked by ectopic PKAC, which is also expressed in the nucleus.


This item appears in the following Collection(s)

Show simple item record