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    Hepatocyte growth factor/scatter factor has distinct classes of binding site in heparan sulfate from mammary cells.

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    Authors
    Rahmoune, H
    Rudland, P S
    Gallagher, John T
    Fernig, D G
    Affiliation
    School of Biological Sciences, Life Sciences Building, University of Liverpool, U.K.
    Issue Date
    1998-04-28
    
    Metadata
    Show full item record
    Abstract
    Hepatocyte growth factor/scatter factor (HGF/SF) is a heparan sulfate (HS)-binding growth factor and morphogen for mammary epithelial cells that is produced by mammary stromal fibroblasts. HS chains, purified as peptidoglycans from a panel of cell lines representative of the ductal epithelial cell (Huma 123), the myoepithelial cell (Huma 109), the stromal fibroblast (Rama 27), and malignant mammary epithelial cells (MCF-7 and ZR-75), were used in a biosensor-based assay to identify the classes of HGF/SF-binding sites in the polysaccharide chains. At least three distinct binding sites were identified. One site exhibits fast association and fast dissociation kinetics [kass (1.4-7.7) x 10(6) M-1 s-1; kdiss 0. 0032-0.0096 s-1] and is present on the HS from benign Huma 123 epithelial cells, Huma 109 myoepithelial-like cells, and ZR-75 malignant cells. The second binding site, found on HS from the malignant MCF-7 cells, has slower HGF/SF-binding kinetics (kass 0.20 x 10(6) M-1 s-1; kdiss 0.00055 s-1). The third binding site possesses fast association and slow dissociation kinetics (kass 1.1 x 10(6) M-1 s-1; kdiss 0.00020 s-1) and was found on the HS isolated from the culture medium of the Huma 123 benign epithelial cells. The first and second binding sites have a similar Kd, 1-3 nM, while the third binding site has a considerably higher affinity for HGF/SF (Kd 200 pM). The three binding sites seem to be mutually exclusive, since each sample of HS possessed just one of the sites.
    Citation
    Hepatocyte growth factor/scatter factor has distinct classes of binding site in heparan sulfate from mammary cells. 1998, 37 (17):6003-8 Biochemistry
    Journal
    Biochemistry
    URI
    http://hdl.handle.net/10541/92754
    DOI
    10.1021/bi972468t
    PubMed ID
    9558337
    Type
    Article
    Language
    en
    ISSN
    0006-2960
    ae974a485f413a2113503eed53cd6c53
    10.1021/bi972468t
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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