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dc.contributor.authorScott, David
dc.contributor.authorBarber, J B
dc.contributor.authorLevine, Edward
dc.contributor.authorBurrill, W
dc.contributor.authorRoberts, Stephen A
dc.date.accessioned2010-02-17T12:00:50Z
dc.date.available2010-02-17T12:00:50Z
dc.date.issued1998-02
dc.identifier.citationRadiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition? 1998, 77 (4):614-20 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid9484819
dc.identifier.urihttp://hdl.handle.net/10541/92344
dc.description.abstractEnhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBreast Neoplasms
dc.subject.meshCell Division
dc.subject.meshConfounding Factors (Epidemiology)
dc.subject.meshDisease Susceptibility
dc.subject.meshFemale
dc.subject.meshG0 Phase
dc.subject.meshHumans
dc.subject.meshLymphocytes
dc.subject.meshMicronucleus Tests
dc.subject.meshMiddle Aged
dc.subject.meshRadiation Dosage
dc.subject.meshReproducibility of Results
dc.titleRadiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition?en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie CRC Research Centre, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractEnhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. We previously showed that 42% of an unselected series of breast cancer patients and 9% of healthy control subjects showed elevated chromosomal radiosensitivity of lymphocytes irradiated in the G2 phase of the cell cycle. We suggested that, in addition to the highly penetrant genes BRCA1 and BRCA2, which confer a very high risk of breast cancer and are carried by about 5% of all breast cancer patients, there are also low-penetrance predisposing genes carried by a much higher proportion of breast cancer patients, a view supported by recent epidemiological studies. Ideally, testing for the presence of these putative genes should involve the use of simpler methods than the G2 assay, which requires metaphase analysis of chromosome damage. Here we report on the use of a simple, rapid micronucleus assay in G0 lymphocytes exposed to high dose rate (HDR) or low dose rate gamma-irradiation, with delayed mitogenic stimulation. Good assay reproducibility was obtained, particularly with the HDR protocol, which identified 31% (12 out of 39) of breast cancer patients compared with 5% (2 out of 42) of healthy controls as having elevated radiation sensitivity. In the long term, such cytogenetic assays may have the potential for selecting women for intensive screening for breast cancer.


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