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dc.contributor.authorHerring, C J
dc.contributor.authorWest, Catharine M L
dc.contributor.authorWilks, Deepti P
dc.contributor.authorDavidson, Susan E
dc.contributor.authorHunter, Robin D
dc.contributor.authorBerry, P
dc.contributor.authorForster, Gill
dc.contributor.authorMacKinnon, J
dc.contributor.authorRafferty, Joseph A
dc.contributor.authorElder, Rhoderick H
dc.contributor.authorHendry, Jolyon H
dc.contributor.authorMargison, Geoffrey P
dc.date.accessioned2010-02-12T16:15:32Z
dc.date.available2010-02-12T16:15:32Z
dc.date.issued1998-11
dc.identifier.citationLevels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers. 1998, 78 (9):1128-33 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid9820167
dc.identifier.urihttp://hdl.handle.net/10541/92037
dc.description.abstractA study was made of the relationship between the intrinsic radiosensitivity of human cervical tumours and the expression of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (HAP1). The radiosensitivity of clonogenic cells in tumour biopsies was measured as surviving fraction at 2 Gy (SF2) using a soft agar assay. HAP1 expression levels were determined after staining of formalin-fixed paraffin-embedded tumour sections with a rabbit antiserum raised against recombinant HAP1. Both measurements were obtained on pretreatment biopsy material. All 25 tumours examined showed positive staining for HAP1, but there was heterogeneity in the level of expression both within and between tumours. The average coefficients of variation for intra- and intertumour heterogeneity were 62% and 82% respectively. There was a moderate but significant positive correlation between the levels of HAP1 expression and SF2 (r = 0.60, P = 0.002). Hence, this study shows that there is some relationship between intrinsic radiosensitivity and expression of a DNA repair enzyme in cervical carcinomas. The results suggest that this type of approach may be useful in the development of rapid predictive tests of tumour radiosensitivity.
dc.language.isoenen
dc.subjectUterine Cervical Canceren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAnimals
dc.subject.meshCHO Cells
dc.subject.meshCarbon-Oxygen Lyases
dc.subject.meshCricetinae
dc.subject.meshDNA Repair
dc.subject.meshDNA-(Apurinic or Apyrimidinic Site) Lyase
dc.subject.meshDeoxyribonuclease IV (Phage T4-Induced)
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshNuclear Proteins
dc.subject.meshRabbits
dc.subject.meshRadiation Tolerance
dc.subject.meshRats
dc.subject.meshTumor Suppressor Protein p53
dc.subject.meshUterine Cervical Neoplasms
dc.titleLevels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractA study was made of the relationship between the intrinsic radiosensitivity of human cervical tumours and the expression of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (HAP1). The radiosensitivity of clonogenic cells in tumour biopsies was measured as surviving fraction at 2 Gy (SF2) using a soft agar assay. HAP1 expression levels were determined after staining of formalin-fixed paraffin-embedded tumour sections with a rabbit antiserum raised against recombinant HAP1. Both measurements were obtained on pretreatment biopsy material. All 25 tumours examined showed positive staining for HAP1, but there was heterogeneity in the level of expression both within and between tumours. The average coefficients of variation for intra- and intertumour heterogeneity were 62% and 82% respectively. There was a moderate but significant positive correlation between the levels of HAP1 expression and SF2 (r = 0.60, P = 0.002). Hence, this study shows that there is some relationship between intrinsic radiosensitivity and expression of a DNA repair enzyme in cervical carcinomas. The results suggest that this type of approach may be useful in the development of rapid predictive tests of tumour radiosensitivity.


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