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    Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma.

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    Authors
    Lang, Shona H
    Clarke, Noel W
    George, N J
    Allen, Terence D
    Testa, Nydia G
    Affiliation
    Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital National Health Service Trust, Manchester, UK. shl2@york.ac.uk
    Issue Date
    1998-02-15
    
    Metadata
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    Abstract
    BACKGROUND: Metastases of prostate cancer form selectively within the skeleton. To understand this metastatic spread, we studied the ability of prostate epithelial cells to grow and proliferate within the bone marrow, using primary coculture. METHODS: Prostate epithelia and fibroblasts were prepared from men with benign prostatic hyperplasia (n = 13) and cancer of the prostate (n = 10). Confluent cultures of bone-marrow stroma or fibroblast controls were prepared in 96-well plates, and identical plates were treated with detergent to expose the extracellular matrix of the cells. Epithelial cells were seeded onto either cells or matrix, and their growth characteristics were determined by counting increases in colony size and number over time. Further experiments evaluated the effects on epithelial growth when cells were exposed to media conditioned by these stroma, using an MTT assay. RESULTS: Results showed that for epithelial cells derived from malignant (or benign) tissue, the median value of the total area of colonies formed on bone-marrow stroma was 2.1 (benign, 2.6) mm2, in contrast to 0.3 (benign, 0.4) mm2 or 0.25 (benign, 0) mm2 when these cells were cocultured with fibroblasts from benign or malignant prostates, respectively. Statistics indicated that growth was significantly greater on bone-marrow stroma than on control stroma (P < 0.005). However, no significant stimulation of epithelial cell growth was seen when these epithelial cells were cultured on extracellular matrix from bone-marrow stroma or when exposed to bone-marrow stroma-conditioned media in comparison to fibroblast controls. No statistical differences were found between the formation of colonies from malignant tissue in comparison to benign. CONCLUSIONS: This system allows the investigation of bone-marrow stroma colonization by primary prostate epithelial cells, and could be developed for the study of metastasis.
    Citation
    Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma. 1998, 34 (3):203-13 Prostate
    Journal
    Prostate
    URI
    http://hdl.handle.net/10541/92031
    DOI
    10.1002/(SICI)1097-0045(19980215)34:3<203::AID-PROS8>3.0.CO;2-I
    PubMed ID
    9492849
    Type
    Article
    Language
    en
    ISSN
    0270-4137
    ae974a485f413a2113503eed53cd6c53
    10.1002/(SICI)1097-0045(19980215)34:3<203::AID-PROS8>3.0.CO;2-I
    Scopus Count
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    All Paterson Institute for Cancer Research

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