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    High-performance liquid chromatographic assay for the novel antitumor drug, bryostatin-1, incorporating a serum extraction technique.

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    Authors
    Khan, Parveen
    McGown, Alan T
    Dawson, Martin J
    Jayson, Gordon C
    Prendiville, Joseph A
    Pettit, G R
    Crowther, Derek
    Affiliation
    CRC Department of Experimental Chemotherapy, Paterson Institute for Cancer Research, Christie CRC Cancer Centre, Manchester, UK.
    Issue Date
    1998-05-08
    
    Metadata
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    Abstract
    An HPLC assay incorporating a solid-phase extraction technique has been devised for bryostatin-1. Quantitation of bryostatin was found to be linear over the concentration range 0.012-25 microg/ml (0.2-25 ng on column) and was found to have a limit of detection of 0.2 ng on column, with a correlation coefficient of 0.9999. Following extraction of bryostatin over a range of concentrations from horse serum (0.012-25 microg/ml) and human serum (0.01-0.32 microg/ml) using a 100-mg C18 solid-phase extraction cartridge, extraction efficiencies consistently greater than 90% were obtained for extraction from horse serum and varied between 57 and 85% from human serum. However, on extending this work to blood samples from patients undergoing therapy with bryostatin-1, the drug was not detectable even at the maximum dose given, demonstrating the rapid loss of this agent from peripheral circulation.
    Citation
    High-performance liquid chromatographic assay for the novel antitumor drug, bryostatin-1, incorporating a serum extraction technique. 1998, 709 (1):113-7 J. Chromatogr. B Biomed. Sci. Appl.
    Journal
    Journal of Chromatography. B, Biomedical Sciences and Applications
    URI
    http://hdl.handle.net/10541/92012
    DOI
    10.1016/S0378-4347(98)00007-3
    PubMed ID
    9653932
    Type
    Article
    Language
    en
    ISSN
    1387-2273
    ae974a485f413a2113503eed53cd6c53
    10.1016/S0378-4347(98)00007-3
    Scopus Count
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

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