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dc.contributor.authorYamazaki, K
dc.contributor.authorEyden, Brian P
dc.date.accessioned2010-02-12T14:39:31Z
dc.date.available2010-02-12T14:39:31Z
dc.date.issued1998-04
dc.identifier.citationCharacterisation of breast stromal fibroblasts: cell surface distribution of collagen type IV, laminin and fibronectin. 1998, 30 (2):217-26 J. Submicrosc. Cytol. Pathol.en
dc.identifier.issn1122-9497
dc.identifier.pmid9648285
dc.identifier.urihttp://hdl.handle.net/10541/91984
dc.description.abstractA number of cells--fibroblasts, chondrocytes and osteoblasts for example--lack the conspicuous cell surface specialisation known as lamina: instead, they possess subplasmalemmal linear densities (SLDs). These have been documented ultrastructurally as having a lamina-like external component but the extent to which they resemble true lamina in terms of protein composition has not been investigated. The relationship of the external component of the SLD to true lamina was examined in this study by light microscope immunostaining, conventional transmission electron microscopy and immuno-electronmicroscopy in intralobular stromal fibroblasts. These were studied in normal peri-tumoral breast tissue in 17 patients undergoing surgery for breast lesions. For ultrastructural immunostaining the indirect immunoperoxidase procedure was used on cryostat sections followed by embedding in epoxy resin. To varying degrees, collagen type IV, laminin and fibronectin antibodies stained fibroblasts and macrophages at the light microscope level. Using immuno-electronmicroscopy, all three antibodies localised as foci on fibroblast and macrophage surfaces. These occurred with a frequency comparable to that of SLDs as seen in non-immunostaining ultrathin sections. These observations represent a first attempt to define the protein composition of SLDs in fibroblasts in vivo. They provide an opportunity of comparing these structures with true lamina and form a basis for understanding how fibroblasts interact with their environment.
dc.language.isoenen
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBreast
dc.subject.meshCollagen
dc.subject.meshFemale
dc.subject.meshFibroblasts
dc.subject.meshFibronectins
dc.subject.meshHumans
dc.subject.meshImmunoenzyme Techniques
dc.subject.meshLaminin
dc.subject.meshMicroscopy, Electron
dc.subject.meshMicroscopy, Immunoelectron
dc.subject.meshMiddle Aged
dc.subject.meshStromal Cells
dc.titleCharacterisation of breast stromal fibroblasts: cell surface distribution of collagen type IV, laminin and fibronectin.en
dc.typeArticleen
dc.contributor.departmentDepartment of Diagnostic Pathology, School of Medicine Keio University, Tokyo, Japan.en
dc.identifier.journalJournal of Submicroscopic Cytology and Pathologyen
html.description.abstractA number of cells--fibroblasts, chondrocytes and osteoblasts for example--lack the conspicuous cell surface specialisation known as lamina: instead, they possess subplasmalemmal linear densities (SLDs). These have been documented ultrastructurally as having a lamina-like external component but the extent to which they resemble true lamina in terms of protein composition has not been investigated. The relationship of the external component of the SLD to true lamina was examined in this study by light microscope immunostaining, conventional transmission electron microscopy and immuno-electronmicroscopy in intralobular stromal fibroblasts. These were studied in normal peri-tumoral breast tissue in 17 patients undergoing surgery for breast lesions. For ultrastructural immunostaining the indirect immunoperoxidase procedure was used on cryostat sections followed by embedding in epoxy resin. To varying degrees, collagen type IV, laminin and fibronectin antibodies stained fibroblasts and macrophages at the light microscope level. Using immuno-electronmicroscopy, all three antibodies localised as foci on fibroblast and macrophage surfaces. These occurred with a frequency comparable to that of SLDs as seen in non-immunostaining ultrathin sections. These observations represent a first attempt to define the protein composition of SLDs in fibroblasts in vivo. They provide an opportunity of comparing these structures with true lamina and form a basis for understanding how fibroblasts interact with their environment.


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