Comparative genomic hybridization as a tool in tumour cytogenetics.
Authors
James, Louise AAffiliation
CRC Section of Molecular Genetics, Paterson Institute for Cancer Research, Wilmslow Road, Manchester, M20 9BX, UK.Issue Date
1999-03
Metadata
Show full item recordAbstract
The quality of cytogenetic analysis of solid tumours has greatly improved in the past decade, but a number of technical difficulties remain which limit the characterization of solid tumour chromosomes by conventional cytogenetics alone. The identification of regions of chromosomal abnormality has been aided by the introduction of molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH). Of these, a recently developed approach, comparative genomic hybridization (CGH), has had a particular impact on the cytogenetic analysis of solid tumours. It incorporates the sensitivity of in situ techniques and overcomes many of the drawbacks of conventional cytogenetic analysis. This review first outlines the CGH method, giving details for the preparation of DNA probes and target human metaphase chromosomes together with information on the in situ technique and data handling criteria used in our laboratory. It then presents an overview of some of the current applications of CGH, together with a discussion of future directions in the field.Citation
Comparative genomic hybridization as a tool in tumour cytogenetics. 1999, 187 (4):385-95 J. Pathol.Journal
Journal of PathologyDOI
10.1002/(SICI)1096-9896(199903)187:4<385::AID-PATH290>3.0.CO;2-5PubMed ID
10398096Type
ArticleLanguage
enISSN
0022-3417ae974a485f413a2113503eed53cd6c53
10.1002/(SICI)1096-9896(199903)187:4<385::AID-PATH290>3.0.CO;2-5