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    Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.

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    Authors
    Drummond, Sheona P
    Ferrigno, Paul
    Lyon, Carol
    Murphy, Jackie
    Goldberg, Martin W
    Allen, Terence D
    Smythe, Carl
    Hutchison, Christopher J
    Affiliation
    MRC Protein Phosphorylation Unit, University of Dundee, Dundee DD1 4HN, Scotland, United Kingdom.
    Issue Date
    1999-01-25
    
    Metadata
    Show full item record
    Abstract
    In this work, we have used novel mAbs against two proteins of the endoplasmic reticulum and outer nuclear membrane, termed NEP-B78 and p65, in addition to a polyclonal antibody against the inner nuclear membrane protein LBR (lamin B receptor), to study the order and dynamics of NE reassembly in the Xenopus cell-free system. Using these reagents, we demonstrate differences in the timing of recruitment of their cognate membrane proteins to the surface of decondensing chromatin in both the cell-free system and XLK-2 cells. We show unequivocally that, in the cell-free system, two functionally and biochemically distinct vesicle types are necessary for NE assembly. We find that the process of distinct vesicle recruitment to chromatin is an ordered one and that NEP-B78 defines a vesicle population involved in the earliest events of reassembly in this system. Finally, we present evidence that NEP-B78 may be required for the targeting of these vesicles to the surface of decondensing chromatin in this system. The results have important implications for the understanding of the mechanisms of nuclear envelope disassembly and reassembly during mitosis and for the development of systems to identify novel molecules that control these processes.
    Citation
    Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types. 1999, 144 (2):225-40 J. Cell Biol.
    Journal
    The Journal of Cell Biology
    URI
    http://hdl.handle.net/10541/91350
    PubMed ID
    9922450
    Type
    Article
    Language
    en
    ISSN
    0021-9525
    Collections
    All Paterson Institute for Cancer Research

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