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dc.contributor.authorReipert, Siegfried
dc.contributor.authorBennion, Gordon
dc.contributor.authorHickman, John A
dc.contributor.authorAllen, Terence D
dc.date.accessioned2010-01-28T12:01:49Z
dc.date.available2010-01-28T12:01:49Z
dc.date.issued1999-04
dc.identifier.citationNucleolar segregation during apoptosis of haemopoietic stem cell line FDCP-Mix. 1999, 6 (4):334-41 Cell Death Differ.en
dc.identifier.issn1350-9047
dc.identifier.pmid10381627
dc.identifier.doi10.1038/sj.cdd.4400500
dc.identifier.urihttp://hdl.handle.net/10541/90799
dc.description.abstractThe programmed elimination of cells during apoptosis is distinct from necrosis both morphologically and biochemically. Currently, the morphological description of apoptosis discriminates between the segregation of the nucleolus and the so called 'chromatin condensation'. The latter originates from observations of electron dense material adjacent to the nuclear envelope of apoptotic nuclei. Although there is ample evidence for an involvement of DNA in electron dense marginations, their true nature is still unknown. By studying apoptosis in FDCP-Mix, a pluripotent murine haemopoietic stem cell line, we found morphological and histochemical evidence that electron dense material at the nuclear envelope (NE) has emerged as a result of the segregation of nucleoli in association with the nuclear membrane. The remaining electron dense and homogenous bulk of the nucleolus labels for RNAse-gold, but even more intensely for DNAse-gold, and therefore could possibly be mistaken as 'condensed chromatin' in the light microscope. The labelling of the electron dense material for DNase-gold in FDCP-Mix could be explained by a migration of DNA into the bulk of the nucleoli at an early stage of cell death.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshApoptosis
dc.subject.meshCell Line
dc.subject.meshCell Nucleolus
dc.subject.meshChromatin
dc.subject.meshDeoxyribonucleases
dc.subject.meshFlow Cytometry
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshImmunohistochemistry
dc.subject.meshMice
dc.subject.meshMicroscopy, Immunoelectron
dc.subject.meshNuclear Envelope
dc.titleNucleolar segregation during apoptosis of haemopoietic stem cell line FDCP-Mix.en
dc.typeArticleen
dc.contributor.departmentDepartment of Structural Cell Biology, Paterson Institute for Cancer Research & Christie Hospital, Manchester, UK.en
dc.identifier.journalCell Death and Differentiationen
html.description.abstractThe programmed elimination of cells during apoptosis is distinct from necrosis both morphologically and biochemically. Currently, the morphological description of apoptosis discriminates between the segregation of the nucleolus and the so called 'chromatin condensation'. The latter originates from observations of electron dense material adjacent to the nuclear envelope of apoptotic nuclei. Although there is ample evidence for an involvement of DNA in electron dense marginations, their true nature is still unknown. By studying apoptosis in FDCP-Mix, a pluripotent murine haemopoietic stem cell line, we found morphological and histochemical evidence that electron dense material at the nuclear envelope (NE) has emerged as a result of the segregation of nucleoli in association with the nuclear membrane. The remaining electron dense and homogenous bulk of the nucleolus labels for RNAse-gold, but even more intensely for DNAse-gold, and therefore could possibly be mistaken as 'condensed chromatin' in the light microscope. The labelling of the electron dense material for DNase-gold in FDCP-Mix could be explained by a migration of DNA into the bulk of the nucleoli at an early stage of cell death.


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