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    Nucleolar segregation during apoptosis of haemopoietic stem cell line FDCP-Mix.

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    Authors
    Reipert, Siegfried
    Bennion, Gordon
    Hickman, John A
    Allen, Terence D
    Affiliation
    Department of Structural Cell Biology, Paterson Institute for Cancer Research & Christie Hospital, Manchester, UK.
    Issue Date
    1999-04
    
    Metadata
    Show full item record
    Abstract
    The programmed elimination of cells during apoptosis is distinct from necrosis both morphologically and biochemically. Currently, the morphological description of apoptosis discriminates between the segregation of the nucleolus and the so called 'chromatin condensation'. The latter originates from observations of electron dense material adjacent to the nuclear envelope of apoptotic nuclei. Although there is ample evidence for an involvement of DNA in electron dense marginations, their true nature is still unknown. By studying apoptosis in FDCP-Mix, a pluripotent murine haemopoietic stem cell line, we found morphological and histochemical evidence that electron dense material at the nuclear envelope (NE) has emerged as a result of the segregation of nucleoli in association with the nuclear membrane. The remaining electron dense and homogenous bulk of the nucleolus labels for RNAse-gold, but even more intensely for DNAse-gold, and therefore could possibly be mistaken as 'condensed chromatin' in the light microscope. The labelling of the electron dense material for DNase-gold in FDCP-Mix could be explained by a migration of DNA into the bulk of the nucleoli at an early stage of cell death.
    Citation
    Nucleolar segregation during apoptosis of haemopoietic stem cell line FDCP-Mix. 1999, 6 (4):334-41 Cell Death Differ.
    Journal
    Cell Death and Differentiation
    URI
    http://hdl.handle.net/10541/90799
    DOI
    10.1038/sj.cdd.4400500
    PubMed ID
    10381627
    Type
    Article
    Language
    en
    ISSN
    1350-9047
    ae974a485f413a2113503eed53cd6c53
    10.1038/sj.cdd.4400500
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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