Heparin is a unique marker of progenitors in the glial cell lineage.
AffiliationCancer Research Campaign, Paterson Institute of Cancer Research, Christie Hospital, Wilmslow Road, Manchester M20 4BX, United Kingdom. email@example.com
MetadataShow full item record
AbstractThe oligodendrocyte-type-2 astrocyte progenitor cells (precursors of oligodendrocytes and type-2 astrocytes) are an excellent system in which to study differentiation as they can be manipulated in vitro. Maintenance of oligodendrocyte-type-2 astrocyte progenitor cells requires basic fibroblast growth factor, a growth factor whose action normally depends on a heparan sulfate coreceptor. Biochemical analysis revealed a most surprising result: that the oligodendrocyte-type-2 astrocyte progenitors did not synthesize heparan sulfate, the near ubiquitous N-sulfated cell surface polysaccharide, but the chemically related heparin in a form that was almost completely N- and O-sulfated. The heparin was detected in the pericellular fraction of the cells and the culture medium. In contrast the differentiated glial subpopulations (oligodendrocytes and type-2 astrocytes) synthesized typical heparan sulfate but with distinctive fine structural features for each cell type. Thus heparin is a unique differentiation marker in the glial lineage. Previously heparin has been found only in a subset of mature mast cells called the connective tissue mast cells. Its presence within the developing nervous system on a precise population of progenitors may confer specific and essential recognition properties on those cells in relation to binding soluble growth and/or differentiation factors and the extracellular matrix.
CitationHeparin is a unique marker of progenitors in the glial cell lineage. 1999, 274 (36):25455-60 J. Biol. Chem.
JournalThe Journal of Biological Chemistry
- Syndecan-3 and perlecan are differentially expressed by progenitors and mature oligodendrocytes and accumulate in the extracellular matrix.
- Authors: Winkler S, Stahl RC, Carey DJ, Bansal R
- Issue date: 2002 Aug 15
- Heterotypic and homotypic cellular interactions influencing the growth and differentiation of bipotential oligodendrocyte-type-2 astrocyte progenitors in culture.
- Authors: Agresti C, Aloisi F, Levi G
- Issue date: 1991 Mar
- Control of division and differentiation in oligodendrocyte-type-2 astrocyte progenitor cells.
- Authors: Noble M, Barnett SC, Bögler O, Land H, Wolswijk G, Wren D
- Issue date: 1990
- The effect of oncogenes on the growth and differentiation of oligodendrocyte type 2 astrocyte progenitor cells.
- Authors: Barnett SC, Crouch DH
- Issue date: 1995 Jan
- Cooperation between two growth factors promotes extended self-renewal and inhibits differentiation of oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells.
- Authors: Bögler O, Wren D, Barnett SC, Land H, Noble M
- Issue date: 1990 Aug