Photoprotection by topical DNA repair enzymes: molecular correlates of clinical studies.
Affiliation
AGI Dermatics, Freeport, NY 11420, USA. agiderm@aol.comIssue Date
1999-02
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A new approach to photoprotection is to repair DNA damage after UV exposure. This can be accomplished by delivery of a DNA repair enzyme with specificity to UV-induced cyclobutane pyrimidine dimers into skin by means of specially engineered liposomes. Treatment of DNA-repair-deficient xeroderma pigmentosum patients or skin cancer patients with T4N5 liposome lotion containing such DNA repair liposomes increases the removal of DNA damage in the first few hours after treatment. In these studies, a DNA repair effect was observed in some patients treated with heat-inactivated enzyme. Unexpectedly, it was discovered that the heat-inactivated T4 endonuclease V enzyme refolds and recovers enzymatic activity. These studies demonstrate that measurements of molecular changes induced by biological drugs are useful adjuvants to clinical studies.Citation
Photoprotection by topical DNA repair enzymes: molecular correlates of clinical studies. 1999, 69 (2):136-40 Photochem. Photobiol.Journal
Photochemistry and PhotobiologyDOI
10.1111/j.1751-1097.1999.tb03265.xPubMed ID
10048308Type
ArticleLanguage
enISSN
0031-8655ae974a485f413a2113503eed53cd6c53
10.1111/j.1751-1097.1999.tb03265.x
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