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dc.contributor.authorThomson, P J
dc.contributor.authorPotten, Christopher S
dc.contributor.authorAppleton, D R
dc.date.accessioned2010-01-28T12:08:08Z
dc.date.available2010-01-28T12:08:08Z
dc.date.issued1999-10
dc.identifier.citationMapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis? 1999, 37 (5):377-83 Br J Oral Maxillofac Surgen
dc.identifier.issn0266-4356
dc.identifier.pmid10577752
dc.identifier.doi10.1054/bjom.1999.0130
dc.identifier.urihttp://hdl.handle.net/10541/90765
dc.description.abstractOur aim was to characterize epithelial cell proliferative activity within the oral cavity and to find out if there were differences between sites with high and low incidence of cancer. A total of 105 samples of clinically normal mucosa were harvested from various intra-oral sites. Excised specimens were incubated in vitro with tritiated thymidine and bromodeoxyuridine to 'double label' cells undergoing DNA synthesis, and enable calculation of the duration of S phase and estimation of variables of cell flux to and from S. Mean labelling indices (percentage of cells within the S phase of the cell cycle) were highest in the floor of mouth (12.3%) and ventral tongue (10.1%), while activity was lowest in the dorsum of tongue (4.3%) and the palate (7.2%), P<0.001. In general, both cell influx and the duration of S increased proportionally to the labelling index. Sites with a high incidence of cancer were characterized by high labelling indices, increased cell influx and a prolonged S phase.
dc.language.isoenen
dc.subjectMouth Canceren
dc.subject.meshAdult
dc.subject.meshAnalysis of Variance
dc.subject.meshCell Division
dc.subject.meshCytological Techniques
dc.subject.meshEpithelial Cells
dc.subject.meshHumans
dc.subject.meshMouth Mucosa
dc.subject.meshMouth Neoplasms
dc.subject.meshReference Values
dc.titleMapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis?en
dc.typeArticleen
dc.contributor.departmentDepartment of Oral & Maxillofacial Surgery, The Dental School, University of Newcastle upon Tyne, UK.en
dc.identifier.journalThe British Journal of Oral & Maxillofacial Surgeryen
html.description.abstractOur aim was to characterize epithelial cell proliferative activity within the oral cavity and to find out if there were differences between sites with high and low incidence of cancer. A total of 105 samples of clinically normal mucosa were harvested from various intra-oral sites. Excised specimens were incubated in vitro with tritiated thymidine and bromodeoxyuridine to 'double label' cells undergoing DNA synthesis, and enable calculation of the duration of S phase and estimation of variables of cell flux to and from S. Mean labelling indices (percentage of cells within the S phase of the cell cycle) were highest in the floor of mouth (12.3%) and ventral tongue (10.1%), while activity was lowest in the dorsum of tongue (4.3%) and the palate (7.2%), P<0.001. In general, both cell influx and the duration of S increased proportionally to the labelling index. Sites with a high incidence of cancer were characterized by high labelling indices, increased cell influx and a prolonged S phase.


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