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    Monomer complexes of basic fibroblast growth factor and heparan sulfate oligosaccharides are the minimal functional unit for cell activation.

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    Authors
    Pye, David A
    Gallagher, John T
    Affiliation
    Cancer Research Campaign (CRC) Department of Drug Development and Imaging, Paterson Institute for Cancer Research, Christie Hospital, Manchester M20 4BX, United Kingdom. dype@picr.man.ac.uk
    Issue Date
    1999-05-07
    
    Metadata
    Show full item record
    Abstract
    The interaction of basic fibroblast growth factor (bFGF) with heparan sulfate (HS)/heparin has been shown to strongly enhance the activity of the growth factor although the mechanism of activation is unclear. We have addressed the issue of the minimal stoichiometry of an active HS oligosaccharide.bFGF complex by chemically cross-linking the two components to form novel covalent conjugates. The cross-linking procedure produced both monomeric and dimeric bFGF. oligosaccharide complexes, which were purified to homogeneity. Dimer conjugates were shown to have been formed as a result of disulfide bridging of monomer conjugates. These monomer conjugates were subsequently found to be biologically active in a mitogenesis assay. We therefore conclude that a monomeric bFGF.oligosaccharide complex is the minimal functional unit required for mitogenic stimulation.
    Citation
    Monomer complexes of basic fibroblast growth factor and heparan sulfate oligosaccharides are the minimal functional unit for cell activation. 1999, 274 (19):13456-61 J. Biol. Chem.
    Journal
    Journal of Biological Chemistry
    URI
    http://hdl.handle.net/10541/90762
    DOI
    10.1074/jbc.274.19.13456
    PubMed ID
    10224111
    Type
    Article
    Language
    en
    ISSN
    0021-9258
    ae974a485f413a2113503eed53cd6c53
    10.1074/jbc.274.19.13456
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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