Authors
Björk-Eriksson, TWest, Catharine M L
Karlsson, E
Slevin, Nicholas J
Davidson, Susan E
James, Roger D
Mercke, C
Affiliation
Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.Issue Date
1998-06
Metadata
Show full item recordAbstract
A study was made of the intrinsic radiosensitivity of 140 biopsy and surgical specimens of malignant head and neck tumours of different histologies. Using a soft-agar clonogenic assay, the material was assessed for the ability to grow in culture (colony-forming efficiency; CFE) and inherent tumour radiosensitivity (surviving fraction at 2 Gy, SF2). The success rate for obtaining growth was 74% (104/140) with a mean CFE of 0.093% (median 0.031) and a range of 0.002-1.3%. SF2 was obtained for 88 of 140 specimens, representing a success rate of 63% with a mean SF2 of 0.48 (median 0.43) and a range of 0.10-1.00. There were no significant differences in radiosensitivity between different sites of the head and neck region. There were no significant relationships between SF2 and disease stage, nodal status, tumour grade, patient age, primary tumour growth pattern and CFE. The results were compared with those for other tumour types previously analysed with the same assay. The distribution of the SF2 values for the head and neck tumours was similar to that for 145 cervix carcinomas and there was no significant difference in mean radiosensitivity between the two tumour types. Also, there was no significant difference in radiosensitivity between head and neck tumours and either breast or colorectal cancers. However, a group of eight lymphomas was significantly more radiosensitive. These results confirm the feasibility of carrying out radiosensitivity measurements using a soft-agar clonogenic assay on head and neck tumours. In addition, the work has shown that radiosensitivity is independent of many clinical parameters and that the mean value is similar to that reported for cervix carcinomas.Citation
The in vitro radiosensitivity of human head and neck cancers. 1998, 77 (12):2371-5 Br. J. CancerJournal
British Journal of CancerPubMed ID
9649161Type
ArticleLanguage
enISSN
0007-0920Collections
Related articles
- Relationship between tumour cell in vitro radiosensitivity and clinical outcome after curative radiotherapy for squamous cell carcinoma of the head and neck.
- Authors: Stausbøl-Grøn B, Overgaard J
- Issue date: 1999 Jan
- Tumor radiosensitivity (SF2) is a prognostic factor for local control in head and neck cancers.
- Authors: Björk-Eriksson T, West C, Karlsson E, Mercke C
- Issue date: 2000 Jan 1
- In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data.
- Authors: Stausbøl-Grøn B, Bentzen SM, Jørgensen KE, Nielsen OS, Bundgaard T, Overgaard J
- Issue date: 1999 Mar
- Selective assessment of in vitro radiosensitivity of tumour cells and fibroblasts from single tumour biopsies using immunocytochemical identification of colonies in the soft agar clonogenic assay.
- Authors: Stausbøl-Grøn B, Nielsen OS, Møller Bentzen S, Overgaard J
- Issue date: 1995 Nov
- The lack of correlation between proliferation (Ki-67, PCNA, LI, Tpot), p53 expression and radiosensitivity for head and neck cancers.
- Authors: Björk-Eriksson T, West CM, Cvetskovska E, Svensson M, Karlsson E, Magnusson B, Slevin NJ, Edström S, Mercke C
- Issue date: 1999 Jul