Potential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells.
Authors
Coco Martin, J MMooren, E
Ottenheim, C
Burrill, Wayne
Nunez, M I
Sprong, D
Bartelink, Harry
Begg, Adrian C
Affiliation
Department of Radiotherapy, The Netherlands Cancer Institute, Amsterdam. jose.coco_martin@gist-brocades.infonet.comIssue Date
1999-09
Metadata
Show full item recordAbstract
PURPOSE: To validate whether the number of aberrations could be used as a measure of the radiosensitivity of human tumour cells. If so, this would potentially provide a more rapid method than the colony assay to predict radiocurability in human tumour biopsy material. MATERIALS AND METHODS: A panel of 13 human tumour cell lines was investigated, covering a wide range of radiosensitivities. Fluorescence in situ hybridization (FISH) employing whole chromosome probes was used to detect aberrations. RESULTS: A dose-dependent increase in radiation-induced chromosome aberrations was observed in all cell lines. A good correlation (r=0.90) was found between cell survival and total chromosome aberrations in 12 of the 13 cell lines (92%), with one exception. A poorer correlation was observed between cell survival and stable- (r=0.85) and unstable-type aberrations (r=0.81). Survival-aberration correlations for individual radiation doses were worse, although statistically significant. The exceptional cell line showed significantly more aberrations for a given level of cell kill than expected based on data for the other lines. CONCLUSION: This study indicates that radiation-induced chromosome aberrations can be used as a potential predictor of intrinsic radiosensitivity for the majority of human tumours when more than one dose level is tested. This could aid the design of radiotherapy schedules for each individual patient, or in the decision of whether to use an alternative therapy.Citation
Potential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells. 1999, 75 (9):1161-8 Int. J. Radiat. Biol.Journal
International Journal of Radiation BiologyPubMed ID
10528924Type
ArticleLanguage
enISSN
0955-3002Collections
Related articles
- Use of fluorescence in situ hybridization to measure chromosome aberrations as a predictor of radiosensitivity in human tumour cells.
- Authors: Coco-Martin JM, Smeets MF, Poggensee M, Mooren E, Hofland I, van den Brug M, Ottenheim C, Bartelink H, Begg AC
- Issue date: 1994 Sep
- Relationship between chromosome aberrations, micronuclei and cell kill in two human tumour cell lines of widely differing radiosensitivity.
- Authors: Jones LA, Clegg S, Bush C, McMillan TJ, Peacock JH
- Issue date: 1994 Nov
- Prediction of human cell radiosensitivity: comparison of clonogenic assay with chromosome aberrations scored using premature chromosome condensation with fluorescence in situ hybridization.
- Authors: Sasai K, Evans JW, Kovacs MS, Brown JM
- Issue date: 1994 Dec 1
- Lethality of radiation-induced chromosome aberrations in human tumour cell lines with different radiosensitivities.
- Authors: Coco-Martin JM, Ottenheim CP, Bartelink H, Begg AC
- Issue date: 1996 Mar
- Use of fluorescence in situ hybridization to determine the relationship between chromosome aberrations and cell survival in eight human fibroblast strains.
- Authors: Russell NS, Arlett CF, Bartelink H, Begg AC
- Issue date: 1995 Aug