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dc.contributor.authorBennett, Alice M
dc.contributor.authorLescott, Thelma
dc.contributor.authorPhillpotts, Robert J
dc.contributor.authorMackett, Mike
dc.contributor.authorTitball, Richard W
dc.date.accessioned2010-01-20T16:00:52Z
dc.date.available2010-01-20T16:00:52Z
dc.date.issued1999
dc.identifier.citationRecombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin. 1999, 12 (2):97-105 Viral Immunol.en
dc.identifier.issn0882-8245
dc.identifier.pmid10413356
dc.identifier.urihttp://hdl.handle.net/10541/90173
dc.description.abstractRecombinant vaccinia viruses that expressed the nontoxic C-domain of Clostridium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion sites for the clostridial DNA, and expression of the foreign protein was measured in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the alpha-toxin C-domain were recorded, all of the vaccinia recombinants protected mice against a lethal challenge with alpha-toxin demonstrating that a recombinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBacterial Toxins
dc.subject.meshCalcium-Binding Proteins
dc.subject.meshCell Line
dc.subject.meshCercopithecus aethiops
dc.subject.meshClostridium perfringens
dc.subject.meshFemale
dc.subject.meshGenetic Vectors
dc.subject.meshMice
dc.subject.meshMice, Inbred BALB C
dc.subject.meshRecombination, Genetic
dc.subject.meshType C Phospholipases
dc.subject.meshVaccinia virus
dc.titleRecombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin.en
dc.typeArticleen
dc.contributor.departmentDefence Evaluation and Research Agency, Salisbury, Wiltshire, United Kingdom.en
dc.identifier.journalViral Immunologyen
html.description.abstractRecombinant vaccinia viruses that expressed the nontoxic C-domain of Clostridium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion sites for the clostridial DNA, and expression of the foreign protein was measured in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the alpha-toxin C-domain were recorded, all of the vaccinia recombinants protected mice against a lethal challenge with alpha-toxin demonstrating that a recombinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.


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