Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay.
AffiliationCancer Research Campaign Section of Drug Development and Imaging, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
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AbstractThe single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.
CitationTechnical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay. 2000, 76 (1):61-5 Int. J. Radiat. Biol.
JournalInternational Journal of Radiation Biology
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