Donor stromal cells from human blood engraft in NOD/SCID mice.
dc.contributor.author | Goan, Silvia-Renate | |
dc.contributor.author | Junghahn, Ilse | |
dc.contributor.author | Wissler, Manuela | |
dc.contributor.author | Becker, Michael | |
dc.contributor.author | Aumann, Jutta | |
dc.contributor.author | Just, Ursula | |
dc.contributor.author | Martiny-Baron, Georg | |
dc.contributor.author | Fichtner, Iduna | |
dc.contributor.author | Henschler, Reinhard | |
dc.date.accessioned | 2010-01-19T16:45:40Z | |
dc.date.available | 2010-01-19T16:45:40Z | |
dc.date.issued | 2000-12-01 | |
dc.identifier.citation | Donor stromal cells from human blood engraft in NOD/SCID mice. 2000, 96 (12):3971-8 Blood | en |
dc.identifier.issn | 0006-4971 | |
dc.identifier.pmid | 11090086 | |
dc.identifier.uri | http://hdl.handle.net/10541/90055 | |
dc.description.abstract | Little is known about the presence, frequency, and in vivo proliferative potential of stromal cells within blood-derived hematopoietic transplants. In this study, nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice were injected with human CD34(+) peripheral blood cells (PBCs) or cord blood cells (CBCs, either enriched for CD34 or density-gradient separated mononuclear cells). Flow cytometric analysis 5 to 11 weeks after transplantation revealed the presence of a human lymphomyeloid hematopoiesis within the murine bone marrow. Immunohistochemical staining of bone marrow cell suspensions using human-specific antibodies showed human cells staining positive for human fibroblast markers, human von Willebrand factor (vWF) and human KDR (vascular endothelial growth factor receptor-2) in mice transplanted with CD34(+) PBCs or CBCs, with mean frequencies between 0.6% and 2.4%. In stromal layers of bone marrow cultures established from the mice, immunohistochemical staining using human-specific antibodies revealed flattened reticular cells or spindle-shaped cells staining positive with human-specific antifibroblast antibodies (mean frequency, 2.2%). Cell populations of more rounded cells stained positive with human-specific antibodies recognizing CD34 (1.5%), vWF (2.2%), and KDR (1.6%). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and subsequent complementary DNA sequencing detected transcripts of human KDR (endothelial specific) and human proline hydroxylase-alpha (fibroblast specific) within the bone marrow and spleen of transplanted mice. Analysis of nontransplanted control mice yielded negative results in immunocytochemistry and RT-PCR. Cells expressing endothelial and fibroblast markers were also detected in the grafts before transplantation, and their numbers increased up to 3 log in vivo after transplantation. These results indicate that stromal progenitor cells are present in human cytokine-mobilized peripheral blood or cord blood that engraft in NOD/SCID mice. (Blood. 2000;96:3971-3978) | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Antigens, Surface | |
dc.subject.mesh | Bone Marrow Cells | |
dc.subject.mesh | Cell Culture Techniques | |
dc.subject.mesh | Cell Differentiation | |
dc.subject.mesh | Endothelium | |
dc.subject.mesh | Fibroblasts | |
dc.subject.mesh | Graft Survival | |
dc.subject.mesh | Hematopoiesis | |
dc.subject.mesh | Hematopoietic Stem Cell Transplantation | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Immunohistochemistry | |
dc.subject.mesh | Immunophenotyping | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Mice, Inbred NOD | |
dc.subject.mesh | Mice, SCID | |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | |
dc.subject.mesh | Stromal Cells | |
dc.subject.mesh | Transplantation, Heterologous | |
dc.title | Donor stromal cells from human blood engraft in NOD/SCID mice. | en |
dc.type | Article | en |
dc.contributor.department | Max Delbrück Center for Molecular Medicine, Berlin, Germany. | en |
dc.identifier.journal | Blood | en |
html.description.abstract | Little is known about the presence, frequency, and in vivo proliferative potential of stromal cells within blood-derived hematopoietic transplants. In this study, nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice were injected with human CD34(+) peripheral blood cells (PBCs) or cord blood cells (CBCs, either enriched for CD34 or density-gradient separated mononuclear cells). Flow cytometric analysis 5 to 11 weeks after transplantation revealed the presence of a human lymphomyeloid hematopoiesis within the murine bone marrow. Immunohistochemical staining of bone marrow cell suspensions using human-specific antibodies showed human cells staining positive for human fibroblast markers, human von Willebrand factor (vWF) and human KDR (vascular endothelial growth factor receptor-2) in mice transplanted with CD34(+) PBCs or CBCs, with mean frequencies between 0.6% and 2.4%. In stromal layers of bone marrow cultures established from the mice, immunohistochemical staining using human-specific antibodies revealed flattened reticular cells or spindle-shaped cells staining positive with human-specific antifibroblast antibodies (mean frequency, 2.2%). Cell populations of more rounded cells stained positive with human-specific antibodies recognizing CD34 (1.5%), vWF (2.2%), and KDR (1.6%). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and subsequent complementary DNA sequencing detected transcripts of human KDR (endothelial specific) and human proline hydroxylase-alpha (fibroblast specific) within the bone marrow and spleen of transplanted mice. Analysis of nontransplanted control mice yielded negative results in immunocytochemistry and RT-PCR. Cells expressing endothelial and fibroblast markers were also detected in the grafts before transplantation, and their numbers increased up to 3 log in vivo after transplantation. These results indicate that stromal progenitor cells are present in human cytokine-mobilized peripheral blood or cord blood that engraft in NOD/SCID mice. (Blood. 2000;96:3971-3978) |