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    Bcr-Abl protein tyrosine kinase activity induces a loss of p53 protein that mediates a delay in myeloid differentiation.

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    Authors
    Pierce, Andrew
    Spooncer, Elaine
    Wooley, Sarah
    Dive, Caroline
    Francis, Julia M
    Miyan, Jaleel
    Owen-Lynch, P Jane
    Dexter, T Michael
    Whetton, Anthony D
    Affiliation
    Leukaemia Research Fund Cellular Development Unit, UMIST, Manchester, UK.
    Issue Date
    2000-11-16
    
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    Abstract
    Chronic myeloid leukaemia is a haemopoietic stem cell disorder, the hallmark of which is the expression of the Bcr-Abl Protein Tyrosine Kinase (PTK). We have previously reported that activation of a temperature sensitive Bcr-Abl PTK in the multipotent haemopoietic cell line FDCP-Mix for short periods resulted in subtle changes including, a transient suppression of apoptosis and no inhibition of differentiation. In contrast, activation of the Bcr-Abl PTK for 12 weeks results in cells that display a delay in differentiation at the early granulocyte stage. Flow cytometric analysis also indicates that the expression of cell surface differentiation markers and nuclear morphology are uncoupled. Furthermore, a significant number of the mature neutrophils display abnormal morphological features. Prolonged exposure to Bcr-Abl PTK results in interleukin-3 independent growth and decreased p53 protein levels. FDCP-Mix cells expressing a dominant negative p53 and p53null FDCP-Mix cells demonstrate that the reduction in p53 is causally related to the delay in development. Returning the cells to the restrictive temperature restores the p53 protein levels, the growth factor dependence and largely relieves the effects on development. We conclude that prolonged Bcr-Abl PTK activity within multipotent cells results in a reduction of p53 that drives a delayed and abnormal differentiation.
    Citation
    Bcr-Abl protein tyrosine kinase activity induces a loss of p53 protein that mediates a delay in myeloid differentiation. 2000, 19 (48):5487-97 Oncogene
    Journal
    Oncogene
    URI
    http://hdl.handle.net/10541/90042
    DOI
    10.1038/sj.onc.1203940
    PubMed ID
    11114726
    Type
    Article
    Language
    en
    ISSN
    0950-9232
    ae974a485f413a2113503eed53cd6c53
    10.1038/sj.onc.1203940
    Scopus Count
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