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    Ran alters nuclear pore complex conformation.

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    Authors
    Goldberg, Martin W
    Rutherford, Sandra A
    Hughes, Mike F
    Cotter, Laura A
    Bagley, Steven
    Kiseleva, Elena
    Allen, Terence D
    Clarke, Paul R
    Affiliation
    CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslow Road, Manchester, M20 9BX, UK. mgoldberg@picr.man.ac.uk
    Issue Date
    2000-07-14
    
    Metadata
    Show full item record
    Abstract
    Transport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of the NPC structure suggests a possible active role for it in driving translocation. Here we show that RanGTP but not RanGDP causes alterations of NPC structure when injected into the cytoplasm of Xenopus oocytes, including compaction of the NPC and extension of the cytoplasmic filaments. RanGTP caused accumulation of nucleoplasmin-gold along the length of extended cytoplasmic filaments, whereas RanGDP caused accumulation around the cytoplasmic rim of the NPC. This suggests a possible role for Ran in altering the conformation of the cytoplasmic filaments during transport.
    Citation
    Ran alters nuclear pore complex conformation. 2000, 300 (3):519-29 J. Mol. Biol.
    Journal
    Journal of Molecular Biology
    URI
    http://hdl.handle.net/10541/88046
    DOI
    10.1006/jmbi.2000.3891
    PubMed ID
    10884348
    Type
    Article
    Language
    en
    ISSN
    0022-2836
    ae974a485f413a2113503eed53cd6c53
    10.1006/jmbi.2000.3891
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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