AuthorsGoldberg, Martin W
Rutherford, Sandra A
Hughes, Mike F
Cotter, Laura A
Allen, Terence D
Clarke, Paul R
AffiliationCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslow Road, Manchester, M20 9BX, UK. firstname.lastname@example.org
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AbstractTransport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of the NPC structure suggests a possible active role for it in driving translocation. Here we show that RanGTP but not RanGDP causes alterations of NPC structure when injected into the cytoplasm of Xenopus oocytes, including compaction of the NPC and extension of the cytoplasmic filaments. RanGTP caused accumulation of nucleoplasmin-gold along the length of extended cytoplasmic filaments, whereas RanGDP caused accumulation around the cytoplasmic rim of the NPC. This suggests a possible role for Ran in altering the conformation of the cytoplasmic filaments during transport.
CitationRan alters nuclear pore complex conformation. 2000, 300 (3):519-29 J. Mol. Biol.
JournalJournal of Molecular Biology
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- Issue date: 1996 Oct 15
- The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants.
- Authors: Hughes M, Zhang C, Avis JM, Hutchison CJ, Clarke PR
- Issue date: 1998 Oct
- Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae.
- Authors: Cordes VC, Rackwitz HR, Reidenbach S
- Issue date: 1997 Dec 15
- Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.
- Authors: Rutherford SA, Goldberg MW, Allen TD
- Issue date: 1997 Apr 10
- Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport.
- Authors: Vetter IR, Nowak C, Nishimoto T, Kuhlmann J, Wittinghofer A
- Issue date: 1999 Mar 4