Myeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia.
AuthorsCoignet, Lionel J
Lima, Carmen S
Min, Toon Min
Dyer, Martin J
AffiliationThe Academic Department of Haematology and Cytogenetics, Institute of Cancer Research, Royal Marsden Hospital, Sutton, Surrey, United Kingdom.
MetadataShow full item record
AbstractAbnormalities of chromosome band 13q14 occur in hematologic malignancies of all lineages and at all stages of differentiation. Unlike other chromosomal translocations, which are usually specific for a given lineage, the chromosomal translocation t(12;13)(p12;q14) has been observed in both B-cell and T-cell precursor acute lymphoblastic leukemia (BCP-, TCP-ALL), in differentiated and undifferentiated acute myeloblastic leukemia (AML), and in chronic myeloid leukemia (CML) at progression to blast crisis. The nature of these translocations and their pathologic consequences remain unknown. To begin to define the gene(s) involved on chromosome 13, we have performed fluorescence in situ hybridization (FISH) using a panel of YACs from the region, on a series of 10 cases of acute leukemia with t(12;13)(p12;q14) and 1 case each with "variant" translocations including t(12;13)(q21;q14), t(10;13)(q24;q14) and t(9;13)(p21;q14). In 8/13 cases/cell lines, the 13q14 break fell within a single 1.4 Mb CEPH MegaYAC. This YAC fell immediately telomeric of the forkhead (FKHR) gene, which is disrupted in the t(2;13)(q35;q14) seen in pediatric alveolar rhabdomyosarcoma. Seven of the 8 cases with breaks in this YAC were AML. In 4/13 cases, the 13q14 break fell within a 1.7-Mb YAC located about 3 Mb telomeric of the retinoblastoma (RB1) gene: all 4 cases were ALL. One case of myelodysplastic syndrome exhibited a break within 13q12, adjacent to the BRCA2 gene. These data indicate the presence of myeloid- and lymphoid-specific breakpoint cluster regions within chromosome band 13q14 in acute leukemia.
CitationMyeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia. 1999, 25 (3):222-9 Genes Chromosomes Cancer
JournalGenes, Chromosomes & Cancer
- Identification of complex genomic breakpoint junctions in the t(9;11) MLL-AF9 fusion gene in acute leukemia.
- Authors: Super HG, Strissel PL, Sobulo OM, Burian D, Reshmi SC, Roe B, Zeleznik-Le NJ, Diaz MO, Rowley JD
- Issue date: 1997 Oct
- Analysis of balanced rearrangements of chromosome 6 in acute leukemia: clustered breakpoints in q22-q23 and possible involvement of c-MYB in a new recurrent translocation, t(6;7)(q23;q32 through 36).
- Authors: Sinclair P, Harrison CJ, Jarosová M, Foroni L
- Issue date: 2005 May
- Hematologic malignancies with the t(10;11) (p13;q21) have the same molecular event and a variety of morphologic or immunologic phenotypes.
- Authors: Kobayashi H, Hosoda F, Maseki N, Sakurai M, Imashuku S, Ohki M, Kaneko Y
- Issue date: 1997 Nov
- Aberrations involving 13q12 approximately q14 are frequent secondary events in childhood acute lymphoblastic leukemia.
- Authors: Kovacs BZ, Niggli FK, Betts DR
- Issue date: 2004 Jun
- Hunting 11q23 deletions with fluorescence in situ hybridization (FISH).
- Authors: Cherif D, Bernard O, Paulien S, James MR, Le Paslier D, Berger R
- Issue date: 1994 Apr