• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Lang, Shona H
    Clarke, Noel W
    George, Nicholas J
    Testa, Nydia G
    Affiliation
    CRC Section of Haemopoietic Cell and Gene Therapeutics, Patterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK. shl2@imap.york.ac.uk
    Issue Date
    1999-06
    
    Metadata
    Show full item record
    Abstract
    Prostate cancer metastases form selectively in the bone marrow. Previously we demonstrated motility was important for the formation of primary prostatic epithelial cell colonies in bone marrow stroma (BMS) co-culture. In this study we looked at the influence of motility factors on the colony formation of epithelial cells derived from benign (bPEC) or malignant (mPEC) prostate tissue. After 7 days co-culture we found that anti-scatter factor consistently inhibited prostate epithelial cell colony formation on BMS (7/7 mPEC and 4/7 bPEC samples showed significant inhibition). Antibodies against bFGF and 5T4 did not significantly affect colony formation. Addition of fibroblast conditioned media (derived from benign prostates) to co-cultures stimulated the colony formation of bPEC (170%) and mPEC (252%). This stimulation was eliminated by depletion of SF from the conditioned media. Immunohistochemical staining found c-Met expression in 5/6 bPEC cultures and 7/9 mPEC cultures. When grown in BMS co-culture expression of c-Met was positive in 3/6 bPEC and 2/7 mPEC samples. In conclusion, scatter factor influences the in vitro formation of prostate epithelial cell colonies on BMS co-culture.
    Citation
    Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro. 1999, 17 (4):333-40 Clin. Exp. Metastasis
    Journal
    Clinical & Experimental Metastasis
    URI
    http://hdl.handle.net/10541/88012
    DOI
    10.1023/A:1006696002497
    PubMed ID
    10545020
    Type
    Article
    Language
    en
    ISSN
    0262-0898
    ae974a485f413a2113503eed53cd6c53
    10.1023/A:1006696002497
    Scopus Count
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Interaction of prostate epithelial cells from benign and malignant tumor tissue with bone-marrow stroma.
    • Authors: Lang SH, Clarke NW, George NJ, Allen TD, Testa NG
    • Issue date: 1998 Feb 15
    • Normal and malignant prostate epithelial cells differ in their response to hepatocyte growth factor/scatter factor.
    • Authors: Gmyrek GA, Walburg M, Webb CP, Yu HM, You X, Vaughan ED, Vande Woude GF, Knudsen BS
    • Issue date: 2001 Aug
    • A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia.
    • Authors: Webber MM, Trakul N, Thraves PS, Bello-DeOcampo D, Chu WW, Storto PD, Huard TK, Rhim JS, Williams DE
    • Issue date: 1999 Jul
    • Induction of hepatocyte growth factor in fibroblasts by tumor-derived factors affects invasive growth of tumor cells: in vitro analysis of tumor-stromal interactions.
    • Authors: Nakamura T, Matsumoto K, Kiritoshi A, Tano Y, Nakamura T
    • Issue date: 1997 Aug 1
    • Interactions of human prostatic epithelial cells with bone marrow endothelium: binding and invasion.
    • Authors: Scott LJ, Clarke NW, George NJ, Shanks JH, Testa NG, Lang SH
    • Issue date: 2001 May 18
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.