Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters.
AffiliationCancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
MetadataShow full item record
AbstractA study was made of the neutral comet assay as a potential method for measuring normal cell radiosensitivity. Eleven fibroblast strains were studied comprising nine derived from vaginal biopsies from pretreatment cervical cancer patients and two strains from radiosensitive individuals. DNA double strand break (dsbs) dose-response curves for both initial and residual (20-h repair time) damage were obtained over the dose range 0-240 Gy, with slopes varying 3.2 and 8-fold respectively. Clonogenic cell survival parameters were available for all the cell strains following both high- and low-dose rate irradiation. There were no correlations between the dose-response slope of the initial level of DNA dsbs and parameters that mainly describe the initial portion of clonogenic radiation survival curves (SF2, alpha, D). A significant correlation (r = -0.63, P = 0.04) was found between the extent of residual DNA dsbs and clonogenicity for all 11 fibroblast strains. The parameter showing the highest correlation with fibroblast cell killing (D) for the nine normal fibroblasts alone was the ratio of initial/residual DNA dsb dose-response slope (r = 0.80, P = < 0.01). A significant correlation (r = -0.67, P = 0.03) with clonogenic radiosensitivity was also found for all 11 cell strains when using the ratio of initial/residual DNA dsb damage at a single dose of 180 Gy. This study shows that fibroblast radiosensitivity measured using the neutral comet assay correlates with clonogenic radiation survival parameters, and therefore may have potential value in predictive testing of normal tissue radiosensitivity.
CitationFibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. 1999, 79 (9-10):1366-71 Br. J. Cancer
JournalBritish Journal of Cancer
- Comparison between pulsed-field gel electrophoresis and the comet assay as predictive assays for radiosensitivity in fibroblasts.
- Authors: Sarkaria JN, Bush C, Eady JJ, Peacock JH, Steel GG, Yarnold JR
- Issue date: 1998 Jul
- Relationship between cellular radiosensitivity and DNA damage measured by comet assay in human normal, NBS and AT fibroblasts.
- Authors: Brammer I, Zoller M, Dikomey E
- Issue date: 2001 Sep
- Relationships between clonogenic cell survival, DNA damage and chromosomal radiosensitivity in nine human cervix carcinoma cell lines.
- Authors: Eastham AM, Atkinson J, West CM
- Issue date: 2001 Mar
- The radiosensitivity of human fibroblast cell lines correlates with residual levels of DNA double-strand breaks.
- Authors: Zhou PK, Sproston AR, Marples B, West CM, Margison GP, Hendry JH
- Issue date: 1998 Jun
- Determination of the initial DNA damage and residual DNA damage remaining after 12 hours of repair in eleven cell lines at low doses of irradiation.
- Authors: Roos WP, Binder A, Böhm L
- Issue date: 2000 Nov