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dc.contributor.authorSheridan, M T
dc.contributor.authorWest, Catharine M L
dc.contributor.authorCooper, Rachel A
dc.contributor.authorStratford, Ian J
dc.contributor.authorLogue, John P
dc.contributor.authorDavidson, Susan E
dc.contributor.authorHunter, Robin D
dc.date.accessioned2009-11-23T10:46:30Z
dc.date.available2009-11-23T10:46:30Z
dc.date.issued2000-03
dc.identifier.citationPretreatment apoptosis in carcinoma of the cervix correlates with changes in tumour oxygenation during radiotherapy. 2000, 82 (6):1177-82 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid10735502
dc.identifier.doi10.1054/bjoc.1999.1059
dc.identifier.urihttp://hdl.handle.net/10541/86673
dc.description.abstractA relationship between hypoxia and apoptosis has been identified in vitro and in experimental tumours. The aim of this study was to investigate the relationship between apoptosis, hypoxia and the change in oxygenation during radiotherapy in human squamous cell carcinoma of the cervix. Forty-two patients with locally advanced disease underwent pretreatment evaluation of tumour oxygenation using an Eppendorf computerized microneedle electrode. Twenty-two of these patients also had a second evaluation of tumour oxygenation after receiving 40-45 Gy external beam radiotherapy. Paraffin-embedded histological sections were obtained from random pretreatment biopsies for all 42 patients. Apoptotic index (AI) was quantified by morphology on TUNEL stained sections. No correlation was found between pretreatment measures of AI and either the median pO2 (r = 0.12, P = 0.44) or percentage of values < 5 mmHg (r = -0.02, P = 0.89). A significant positive correlation was found between AI and the change in tumour oxygenation (ratio of pre:post-treatment % values < 5 mmHg) following radiotherapy (r = 0.61, P = 0.002). The lack of correlation between apoptosis and hypoxia may occur because the Eppendorf measures both acute and chronic hypoxia, and the relative ability of acute hypoxia to induce apoptosis is unknown. These results indicate that cell death via apoptosis may be a mechanism of tumour reoxygenation during radiotherapy.
dc.language.isoenen
dc.subjectUterine Cervical Canceren
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAnoxia
dc.subject.meshApoptosis
dc.subject.meshCarcinoma, Squamous Cell
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMiddle Aged
dc.subject.meshOxygen
dc.subject.meshUterine Cervical Neoplasms
dc.titlePretreatment apoptosis in carcinoma of the cervix correlates with changes in tumour oxygenation during radiotherapy.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractA relationship between hypoxia and apoptosis has been identified in vitro and in experimental tumours. The aim of this study was to investigate the relationship between apoptosis, hypoxia and the change in oxygenation during radiotherapy in human squamous cell carcinoma of the cervix. Forty-two patients with locally advanced disease underwent pretreatment evaluation of tumour oxygenation using an Eppendorf computerized microneedle electrode. Twenty-two of these patients also had a second evaluation of tumour oxygenation after receiving 40-45 Gy external beam radiotherapy. Paraffin-embedded histological sections were obtained from random pretreatment biopsies for all 42 patients. Apoptotic index (AI) was quantified by morphology on TUNEL stained sections. No correlation was found between pretreatment measures of AI and either the median pO2 (r = 0.12, P = 0.44) or percentage of values < 5 mmHg (r = -0.02, P = 0.89). A significant positive correlation was found between AI and the change in tumour oxygenation (ratio of pre:post-treatment % values < 5 mmHg) following radiotherapy (r = 0.61, P = 0.002). The lack of correlation between apoptosis and hypoxia may occur because the Eppendorf measures both acute and chronic hypoxia, and the relative ability of acute hypoxia to induce apoptosis is unknown. These results indicate that cell death via apoptosis may be a mechanism of tumour reoxygenation during radiotherapy.


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