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    Multiple mechanisms underlie HLA dysregulation in cervical cancer.

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    Authors
    Brady, C S
    Bartholomew, J S
    Burt, Deborah J
    Duggan-Keen, Margaret F
    Glenville, S
    Telford, Nicholas
    Little, A M
    Davidson, J A
    Jimenez, P
    Ruiz-Cabello, F
    Garrido, F
    Stern, Peter L
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    Affiliation
    CRC Immunology Group, Paterson Institute for Cancer Research, Manchester, United Kingdom.
    Issue Date
    2000-05
    
    Metadata
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    Abstract
    The consistent dysregulation of HLA expression in cervical neoplasia is likely to influence the natural history of the disease and prospects for cell-mediated vaccine therapies. We have studied the underlying mechanisms in eight new cervical cancer cell lines derived from primary tumour biopsies. At least five independent mechanisms leading to changes in HLA expression were seen: HLA class I allelic transcription but no protein; abnormal HLA class I allelic transcription; no HLA-B locus transcription; loss of heterozygosity (LOH); no gammaIFN-mediated upregulation of HLA class I expression, and/or no interferon-gamma (gammaIFN)-mediated HLA class II induction. These were evident in different combinations in 7/8 cell lines showing that multiple, mostly irreversible mechanisms not overridden by gammaIFN, are responsible for HLA dysregulation in cervical neoplasia. Point mutations were responsible for lack of HLA-A2 expression in two cases. In cell line 808, the mutation encodes a stop codon in exon 3; in cell line 778, mutation of the first intron acceptor site leads to use of an alternative AG site in exon 2, resulting in a frameshift and a stop codon after the translation of only 38 amino acids. Tumour cells showing specific HLA class I loss may have selective advantage in the face of tumour-specific cytotoxic T cells (CTL). Such immune escape mechanisms present a major obstacle for the success of CTL-mediated therapies in cervical cancer.
    Citation
    Multiple mechanisms underlie HLA dysregulation in cervical cancer. 2000, 55 (5):401-11 Tissue Antigens
    Journal
    Tissue Antigens
    URI
    http://hdl.handle.net/10541/86668
    DOI
    10.1034/j.1399-0039.2000.550502.x
    PubMed ID
    10885560
    Type
    Article
    Language
    en
    ISSN
    0001-2815
    ae974a485f413a2113503eed53cd6c53
    10.1034/j.1399-0039.2000.550502.x
    Scopus Count
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    All Christie Publications
    All Paterson Institute for Cancer Research

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