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dc.contributor.authorSengupta, P S
dc.contributor.authorMcGown, Alan T
dc.contributor.authorBajaj, V
dc.contributor.authorBlackhall, Fiona H
dc.contributor.authorSwindell, Ric
dc.contributor.authorBromley, Michael
dc.contributor.authorShanks, Jonathan H
dc.contributor.authorWard, Timothy H
dc.contributor.authorBuckley, C H
dc.contributor.authorReynolds, K
dc.contributor.authorSlade, Richard J
dc.contributor.authorJayson, Gordon C
dc.date.accessioned2009-11-23T10:26:21Z
dc.date.available2009-11-23T10:26:21Z
dc.date.issued2000-12
dc.identifier.citationp53 and related proteins in epithelial ovarian cancer. 2000, 36 (18):2317-28 Eur. J. Canceren
dc.identifier.issn0959-8049
dc.identifier.pmid11094305
dc.identifier.doi10.1016/S0959-8049(00)00301-4
dc.identifier.urihttp://hdl.handle.net/10541/86661
dc.description.abstractWe conducted a retrospective immunohistochemical evaluation of the prognostic significance of the expression of p53 and the related proteins Bax, Bcl-2, growth arrest and DNA damage (Gadd45), murine double minute 2 (Mdm2) and p21(WAF1/CIP1) in chemonaive tumours taken from 66 patients with ovarian cancer. Ki-67 expression (a marker of cell proliferation) was also evaluated immunohistochemically, while apoptosis within malignant cells was determined with the terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) assay. The expression of each of the following proteins was significantly associated in the tumours (P < 0.05 unless otherwise stated): Bax with Bcl-2 (P < 0.01); Bax with Mdm2; p21(WAF1/CIP1) with Gadd45 (P < 0.01); p21(WAF1/CIP1) with p53; p53 with Mdm2. Univariate analysis showed that expression of p53, Bax, bulk residual disease and International Federation of Gynecology and Obstetricians (FIGO) stage were all strongly correlated with response to chemotherapy (P < 0.01). Similarly, the FIGO stage and Ki-67 expression (P < 0.01), as well as pathological subtype and bulk residual disease (P < 0.05), were prognostic factors for disease progression. The FIGO stage and Ki-67 expression were significant prognostic factors for overall survival (P < 0.01), with Gadd45 expression and pathological subtype also significant (P < 0.05) in a univariate analysis. Multivariate analysis for response to chemotherapy showed that expression of p53, Bax and FIGO stage were all independent prognostic factors (P < 0.01). The FIGO stage was the most important independent prognostic factor for progression and survival on multivariate analysis (P < 0.01). However, Ki-67 expression was also an independent prognostic factor for disease progression (P < 0.05) and approached significance for survival (P = 0.055). Taken together, these data suggest that determination of Ki-67 expression could supplement established prognostic factors.
dc.language.isoenen
dc.subjectCancer Stagingen
dc.subjectOvarian Canceren
dc.subjectBiological Tumour Markersen
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAnalysis of Variance
dc.subject.meshApoptosis
dc.subject.meshCyclin-Dependent Kinase Inhibitor p21
dc.subject.meshCyclins
dc.subject.meshFemale
dc.subject.meshGenes, bcl-2
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshIn Situ Nick-End Labeling
dc.subject.meshKi-67 Antigen
dc.subject.meshMiddle Aged
dc.subject.meshNeoplasm Staging
dc.subject.meshOvarian Neoplasms
dc.subject.meshPrognosis
dc.subject.meshProto-Oncogene Proteins
dc.subject.meshProto-Oncogene Proteins c-bcl-2
dc.subject.meshRetrospective Studies
dc.subject.meshTumor Markers, Biological
dc.subject.meshTumor Suppressor Protein p53
dc.subject.meshbcl-2-Associated X Protein
dc.titlep53 and related proteins in epithelial ovarian cancer.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Medical Oncology, Christie Hospital National Health Trust, Withington, Manchester, UK.en
dc.identifier.journalEuropean Journal of Canceren
html.description.abstractWe conducted a retrospective immunohistochemical evaluation of the prognostic significance of the expression of p53 and the related proteins Bax, Bcl-2, growth arrest and DNA damage (Gadd45), murine double minute 2 (Mdm2) and p21(WAF1/CIP1) in chemonaive tumours taken from 66 patients with ovarian cancer. Ki-67 expression (a marker of cell proliferation) was also evaluated immunohistochemically, while apoptosis within malignant cells was determined with the terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) assay. The expression of each of the following proteins was significantly associated in the tumours (P < 0.05 unless otherwise stated): Bax with Bcl-2 (P < 0.01); Bax with Mdm2; p21(WAF1/CIP1) with Gadd45 (P < 0.01); p21(WAF1/CIP1) with p53; p53 with Mdm2. Univariate analysis showed that expression of p53, Bax, bulk residual disease and International Federation of Gynecology and Obstetricians (FIGO) stage were all strongly correlated with response to chemotherapy (P < 0.01). Similarly, the FIGO stage and Ki-67 expression (P < 0.01), as well as pathological subtype and bulk residual disease (P < 0.05), were prognostic factors for disease progression. The FIGO stage and Ki-67 expression were significant prognostic factors for overall survival (P < 0.01), with Gadd45 expression and pathological subtype also significant (P < 0.05) in a univariate analysis. Multivariate analysis for response to chemotherapy showed that expression of p53, Bax and FIGO stage were all independent prognostic factors (P < 0.01). The FIGO stage was the most important independent prognostic factor for progression and survival on multivariate analysis (P < 0.01). However, Ki-67 expression was also an independent prognostic factor for disease progression (P < 0.05) and approached significance for survival (P = 0.055). Taken together, these data suggest that determination of Ki-67 expression could supplement established prognostic factors.


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