Authors
Sengupta, P SMcGown, Alan T
Bajaj, V
Blackhall, Fiona H
Swindell, Ric
Bromley, Michael
Shanks, Jonathan H
Ward, Timothy H
Buckley, C H
Reynolds, K
Slade, Richard J
Jayson, Gordon C
Affiliation
Cancer Research Campaign Department of Medical Oncology, Christie Hospital National Health Trust, Withington, Manchester, UK.Issue Date
2000-12
Metadata
Show full item recordAbstract
We conducted a retrospective immunohistochemical evaluation of the prognostic significance of the expression of p53 and the related proteins Bax, Bcl-2, growth arrest and DNA damage (Gadd45), murine double minute 2 (Mdm2) and p21(WAF1/CIP1) in chemonaive tumours taken from 66 patients with ovarian cancer. Ki-67 expression (a marker of cell proliferation) was also evaluated immunohistochemically, while apoptosis within malignant cells was determined with the terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) assay. The expression of each of the following proteins was significantly associated in the tumours (P < 0.05 unless otherwise stated): Bax with Bcl-2 (P < 0.01); Bax with Mdm2; p21(WAF1/CIP1) with Gadd45 (P < 0.01); p21(WAF1/CIP1) with p53; p53 with Mdm2. Univariate analysis showed that expression of p53, Bax, bulk residual disease and International Federation of Gynecology and Obstetricians (FIGO) stage were all strongly correlated with response to chemotherapy (P < 0.01). Similarly, the FIGO stage and Ki-67 expression (P < 0.01), as well as pathological subtype and bulk residual disease (P < 0.05), were prognostic factors for disease progression. The FIGO stage and Ki-67 expression were significant prognostic factors for overall survival (P < 0.01), with Gadd45 expression and pathological subtype also significant (P < 0.05) in a univariate analysis. Multivariate analysis for response to chemotherapy showed that expression of p53, Bax and FIGO stage were all independent prognostic factors (P < 0.01). The FIGO stage was the most important independent prognostic factor for progression and survival on multivariate analysis (P < 0.01). However, Ki-67 expression was also an independent prognostic factor for disease progression (P < 0.05) and approached significance for survival (P = 0.055). Taken together, these data suggest that determination of Ki-67 expression could supplement established prognostic factors.Citation
p53 and related proteins in epithelial ovarian cancer. 2000, 36 (18):2317-28 Eur. J. CancerJournal
European Journal of CancerDOI
10.1016/S0959-8049(00)00301-4PubMed ID
11094305Type
ArticleLanguage
enISSN
0959-8049ae974a485f413a2113503eed53cd6c53
10.1016/S0959-8049(00)00301-4
Scopus Count
Related articles
- Immunohistochemical markers of cell cycle control applied to ovarian and primary peritoneal surface epithelial neoplasms: p21(WAF1/CIP1) predicts survival and good response to platinin-based chemotherapy.
- Authors: Costa MJ, Hansen CL, Walls JE, Scudder SA
- Issue date: 1999 Jun
- Prognostic significance of p21WAF1/CIP1 expression in tongue squamous cell carcinomas.
- Authors: Xie X, Clausen OP, Boysen M
- Issue date: 2002 Aug
- Transcriptional regulation during p21WAF1/CIP1-induced apoptosis in human ovarian cancer cells.
- Authors: Wu Q, Kirschmeier P, Hockenberry T, Yang TY, Brassard DL, Wang L, McClanahan T, Black S, Rizzi G, Musco ML, Mirza A, Liu S
- Issue date: 2002 Sep 27
- The prognostic importance of p53, bcl-2, and bax in early stage epithelial ovarian carcinoma treated with adjuvant chemotherapy.
- Authors: Skirnisdóttir I, Seidal T, Gerdin E, Sorbe B
- Issue date: 2002 May-Jun
- Prognostic significance of apoptosis in synovial sarcoma: correlation with clinicopathologic parameters, cell proliferative activity, and expression of apoptosis-related proteins.
- Authors: Kawauchi S, Fukuda T, Oda Y, Saito T, Oga A, Takeshita M, Yokoyama K, Chuman H, Iwamoto Y, Sasaki K, Tsuneyoshi M
- Issue date: 2000 Jul