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    Irradiation induced expression of CD31, ICAM-1 and VCAM-1 in human microvascular endothelial cells.

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    Authors
    Quarmby, Steven L
    Hunter, Robin D
    Kumar, Shant
    Affiliation
    Department of Pathological Sciences, Medical School, Manchester University M13 9PT, U.K. steven.l.quarmby@man.ac.uk
    Issue Date
    2000
    
    Metadata
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    Abstract
    The adherence and migration of leukocytes through the endothelium of blood vessels is an important early event which occurs in normal tissues following ionizing irradiation but the underlying mechanisms are not fully understood. ICAM-1, VCAM-1 and CD31 are membrane proteins of endothelial cells, mediate this process when the vasculature is exposed to other inflammatory stimuli. In this study, expression of ICAM-1, VCAM-1 and CD31 on human dermal microvascular endothelial cells (HDMECs) at 72 hours post-irradiation using flow cytometry and northern analysis was determined. Dose-dependent increases in the surface expression and mRNA of ICAM-1 and CD31 were observed. In contrast VCAM-1 was practically undetectable on both control and irradiated HDMECs but was strongly expressed in TNF-alpha activated positive control HDMECs. The upregulation in ICAM-1 and CD31 was independent of radiation-induced changes in cell size, number and cell cycle stage. We suggest that ICAM-1 is active over a prolonged period whereas VCAM-1 acts only transiently in leukocyte-endothelial interactions in the irradiated microvasculature. The late upregulation of CD31 is a novel finding and may have a function in radiation-induced leukocyte extravasation, platelet adherence to the vascular wall and abnormal endothelial cell proliferation. Both ICAM-1 and CD31 seem to be therapeutic targets for the amelioration of radiation-induced normal tissue damage.
    Citation
    Irradiation induced expression of CD31, ICAM-1 and VCAM-1 in human microvascular endothelial cells., 20 (5B):3375-81 Anticancer Res.
    Journal
    Anticancer Research
    URI
    http://hdl.handle.net/10541/86658
    PubMed ID
    11131637
    Type
    Article
    Language
    en
    ISSN
    0250-7005
    Collections
    All Christie Publications

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