Fluorescent location of malignant cells in fine needle aspirates.
AffiliationDivision of Biochemistry, School of Biological Sciences, University of Manchester, Manchester M13 9PT, U.K.
MetadataShow full item record
AbstractMalignant cells in fine needle aspirates possess a cell surface protease which can be targeted with fluorescent affinity probes. Cells with active GB exhibit cell surface fluorescence when stained with such affinity probes. The nuclei of all cells on the slides can be counterstained with a nuclear fluorescent stain. Malignant cells are then located by their cell surface fluorescence and their diagnosis confirmed by examining their fluorescent nuclei. Normal cells and benign cells exhibit no cell surface fluorescence and can be ignored. This technique can be used to rapidly select cells of cytological interest in FNA samples obtained routinely and might be adapted for automated screening of FNA.
CitationFluorescent location of malignant cells in fine needle aspirates., 20 (6C):4871-6 Anticancer Res.
- Fluorescent location of tumour cells in fine needle aspirates.
- Authors: Steven FS, Bell J, Ellis IO
- Issue date: 1991 Sep-Oct
- Affinity preparation of a protein inhibitor recognising a cell surface protease.
- Authors: Steven FS, Griffin MM, Bell J, Talbot IC
- Issue date: 1993
- [Detection of hyperdiploid in malignant cells in fine-needle aspirates from lung cancer by fluorescence in situ hybridization].
- Authors: Jia D, Zhang Z, Liu S, Cheng S
- Issue date: 2000 Dec
- Fluorescent location of malignant cells in smears obtained from sputum.
- Authors: Steven FS, Payne PW, Palcic B
- Issue date: 1994 Sep-Oct
- The protective role of a natural inhibitor in the fluorescent location of cells possessing a latent form of cell surface protease.
- Authors: Steven FS, Williams LA
- Issue date: 1990