Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.
dc.contributor.author | Wolf, Peter | |
dc.contributor.author | Maier, Harald | |
dc.contributor.author | Müllegger, Robert R | |
dc.contributor.author | Chadwick, Caroline A | |
dc.contributor.author | Hofmann-Wellenhof, Rainer | |
dc.contributor.author | Soyer, H Peter | |
dc.contributor.author | Hofer, Angelika | |
dc.contributor.author | Smolle, Josef | |
dc.contributor.author | Horn, Michael | |
dc.contributor.author | Cerroni, Lorenzo | |
dc.contributor.author | Yarosh, Daniel | |
dc.contributor.author | Klein, Jonathan | |
dc.contributor.author | Bucana, Corazon | |
dc.contributor.author | Dunner, Kenneth | |
dc.contributor.author | Potten, Christopher S | |
dc.contributor.author | Hönigsmann, Herbert | |
dc.contributor.author | Kerl, Helmut | |
dc.contributor.author | Kripke, Margaret L | |
dc.date.accessioned | 2009-11-18T10:32:39Z | |
dc.date.available | 2009-11-18T10:32:39Z | |
dc.date.issued | 2000-01 | |
dc.identifier.citation | Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha. 2000, 114 (1):149-56 J. Invest. Dermatol. | en |
dc.identifier.issn | 0022-202X | |
dc.identifier.pmid | 10620131 | |
dc.identifier.doi | 10.1046/j.1523-1747.2000.00839.x | |
dc.identifier.uri | http://hdl.handle.net/10541/86375 | |
dc.description.abstract | Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage. | |
dc.language.iso | en | en |
dc.subject | Tumour Necrosis Factor-alpha | en |
dc.subject.mesh | Administration, Topical | |
dc.subject.mesh | Adult | |
dc.subject.mesh | Aged | |
dc.subject.mesh | DNA Ligases | |
dc.subject.mesh | DNA Repair | |
dc.subject.mesh | Deoxyribonuclease (Pyrimidine Dimer) | |
dc.subject.mesh | Drug Carriers | |
dc.subject.mesh | Endodeoxyribonucleases | |
dc.subject.mesh | Female | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Interleukin-10 | |
dc.subject.mesh | Keratinocytes | |
dc.subject.mesh | Langerhans Cells | |
dc.subject.mesh | Liposomes | |
dc.subject.mesh | Male | |
dc.subject.mesh | Microscopy, Electron | |
dc.subject.mesh | Middle Aged | |
dc.subject.mesh | Radiation-Protective Agents | |
dc.subject.mesh | Skin | |
dc.subject.mesh | Time Factors | |
dc.subject.mesh | Tumor Necrosis Factor-alpha | |
dc.subject.mesh | Ultraviolet Rays | |
dc.subject.mesh | Up-Regulation | |
dc.subject.mesh | Viral Proteins | |
dc.title | Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha. | en |
dc.type | Article | en |
dc.contributor.department | Department of Dermatology, Karl Franzens University, Graz, Austria. peter.wolf@kfunigraz.ac.at | en |
dc.identifier.journal | The Journal of Investigative Dermatology | en |
html.description.abstract | Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage. |