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dc.contributor.authorWolf, Peter
dc.contributor.authorMaier, Harald
dc.contributor.authorMüllegger, Robert R
dc.contributor.authorChadwick, Caroline A
dc.contributor.authorHofmann-Wellenhof, Rainer
dc.contributor.authorSoyer, H Peter
dc.contributor.authorHofer, Angelika
dc.contributor.authorSmolle, Josef
dc.contributor.authorHorn, Michael
dc.contributor.authorCerroni, Lorenzo
dc.contributor.authorYarosh, Daniel
dc.contributor.authorKlein, Jonathan
dc.contributor.authorBucana, Corazon
dc.contributor.authorDunner, Kenneth
dc.contributor.authorPotten, Christopher S
dc.contributor.authorHönigsmann, Herbert
dc.contributor.authorKerl, Helmut
dc.contributor.authorKripke, Margaret L
dc.date.accessioned2009-11-18T10:32:39Z
dc.date.available2009-11-18T10:32:39Z
dc.date.issued2000-01
dc.identifier.citationTopical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha. 2000, 114 (1):149-56 J. Invest. Dermatol.en
dc.identifier.issn0022-202X
dc.identifier.pmid10620131
dc.identifier.doi10.1046/j.1523-1747.2000.00839.x
dc.identifier.urihttp://hdl.handle.net/10541/86375
dc.description.abstractExposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.
dc.language.isoenen
dc.subjectTumour Necrosis Factor-alphaen
dc.subject.meshAdministration, Topical
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshDNA Ligases
dc.subject.meshDNA Repair
dc.subject.meshDeoxyribonuclease (Pyrimidine Dimer)
dc.subject.meshDrug Carriers
dc.subject.meshEndodeoxyribonucleases
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshInterleukin-10
dc.subject.meshKeratinocytes
dc.subject.meshLangerhans Cells
dc.subject.meshLiposomes
dc.subject.meshMale
dc.subject.meshMicroscopy, Electron
dc.subject.meshMiddle Aged
dc.subject.meshRadiation-Protective Agents
dc.subject.meshSkin
dc.subject.meshTime Factors
dc.subject.meshTumor Necrosis Factor-alpha
dc.subject.meshUltraviolet Rays
dc.subject.meshUp-Regulation
dc.subject.meshViral Proteins
dc.titleTopical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.en
dc.typeArticleen
dc.contributor.departmentDepartment of Dermatology, Karl Franzens University, Graz, Austria. peter.wolf@kfunigraz.ac.aten
dc.identifier.journalThe Journal of Investigative Dermatologyen
html.description.abstractExposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.


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