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    Detection of individual differences in radiation-induced apoptosis of peripheral blood lymphocytes in normal individuals, ataxia telangiectasia homozygotes and heterozygotes, and breast cancer patients after radiotherapy.

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    Authors
    Barber, James B P
    West, Catharine M L
    Kiltie, Anne E
    Roberts, Stephen A
    Scott, David
    Affiliation
    CRC Section of Molecular Genetics, Paterson Institute for Cancer Research, Christie CRC Research Centre, Manchester M20 9BX, United Kingdom.
    Issue Date
    2000-05
    
    Metadata
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    Abstract
    Quantification of radiation-induced apoptosis in peripheral blood lymphocytes (PBLs) has been proposed as a possible screening test for cancer-prone individuals and also for the prediction of normal tissue responses after radiotherapy. We have used the TUNEL assay (terminal transferase nick-end labeling) 24 h after irradiation with 4 Gy at high dose rate to assess interindividual differences in radiation-induced apoptosis between (1) a panel of normal individuals, (2) ataxia telangiectasia (AT) homozygotes and heterozygotes, and (3) breast cancer patients who had received radiotherapy 8-13 years ago, including a number of patients who had suffered adverse responses to radiation. With this protocol, we show clear differences in radiation-induced apoptosis between individuals, and good reproducibility in the assay. In agreement with previous reports using EBV-transformed lymphoblasts, we show a very poor induction of apoptosis in AT homozygotes and a reduced level in AT heterozygotes compared to normal individuals. A similar reduced level compared to normal individuals was seen in the breast cancer patients. Despite a wide range of values in the breast cancer patients and good reproducibility on repeat samples, there was no correlation of rates of apoptosis with the severity of breast fibrosis, retraction or telangiectasia. The reduced rate of apoptosis observed in the breast cancer cases may be associated with genetic predisposition to breast cancer; however, we conclude that assays of lymphocyte apoptosis are unlikely to be of use in predicting normal tissue tolerance to radiotherapy.
    Citation
    Detection of individual differences in radiation-induced apoptosis of peripheral blood lymphocytes in normal individuals, ataxia telangiectasia homozygotes and heterozygotes, and breast cancer patients after radiotherapy. 2000, 153 (5 Pt 1):570-8 Radiat. Res.
    Journal
    Radiation Research
    URI
    http://hdl.handle.net/10541/86199
    DOI
    10.1667/0033-7587(2000)153[0570:DOIDIR]2.0.CO;2
    PubMed ID
    10790278
    Type
    Article
    Language
    en
    ISSN
    0033-7587
    ae974a485f413a2113503eed53cd6c53
    10.1667/0033-7587(2000)153[0570:DOIDIR]2.0.CO;2
    Scopus Count
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    All Paterson Institute for Cancer Research

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