• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Elevated levels of the pro-carcinogenic adduct, O(6)-methylguanine, in normal DNA from the cancer prone regions of the large bowel.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Povey, Andrew C
    Hall, C Nicholas
    Badawi, A F
    Cooper, Donald P
    O'Connor, Peter J
    Affiliation
    Cancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Manchester, M20 9BX, UK. apovey@fsi.scg.man.ac.uk
    Issue Date
    2000-09
    
    Metadata
    Show full item record
    Abstract
    BACKGROUND: The pro-mutagenic lesion O(6)-methyldeoxyguanosine (O(6)-MedG), a marker of exposure to many N-nitroso compounds (NOC), can be detected in normal and tumour DNA isolated from colorectal tissue. The biological significance of this exposure is, as yet, unknown but in situ NOC formation is bacterially catalysed suggesting that NOC formation and potentially DNA alkylation will vary throughout the large bowel. AIMS: To determine if O(6)-MedG levels in colorectal DNA vary within the large bowel. PATIENTS: We studied 62 men and women undergoing surgery for colorectal tumours in the north west of England. METHODS: O(6)-MedG levels were measured in paired normal and tumour DNA samples. DNA was digested to nucleosides, fractionated by HPLC, and purified O(6)-MedG quantified by a radioimmunoassay. RESULTS: O(6)-MedG was detected in 27 out of a total of 62 (43%) normal DNA samples and in 30 of 58 (52%) tumour DNA samples: it was present at concentrations of <0. 01-0.94 and <0.01-0.151 micromol O(6)-MedG/mol deoxyguanosine for normal and tumour DNA, respectively. Levels of O(6)-MedG in normal, but not tumour, DNA from the proximal colon were lower than those found in DNA from either the sigmoid colon (p=0.03) or rectum (p=0. 05). When the analysis was restricted to samples that contained O(6)-MedG, similar results were obtained in that O(6)-MedG levels in normal DNA were lower in the proximal colon than in the sigmoid colon (p=0.04) or rectum (p=0.03). CONCLUSIONS: DNA alkylation varied within the large bowel possibly due to in situ NOC formation and was highest in areas of the colon and rectum where the highest incidence of large bowel tumours occurs, suggesting that DNA alkylation may play a role in the aetiology of colorectal cancer.
    Citation
    Elevated levels of the pro-carcinogenic adduct, O(6)-methylguanine, in normal DNA from the cancer prone regions of the large bowel. 2000, 47 (3):362-5 Gut
    Journal
    Gut
    URI
    http://hdl.handle.net/10541/86181
    PubMed ID
    10940272
    Type
    Article
    Language
    en
    ISSN
    0017-5749
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Host determinants of DNA alkylation and DNA repair activity in human colorectal tissue: O(6)-methylguanine levels are associated with GSTT1 genotype and O(6)-alkylguanine-DNA alkyltransferase activity with CYP2D6 genotype.
    • Authors: Povey AC, Hall CN, Badawi AF, Cooper DP, Guppy MJ, Jackson PE, O'Connor PJ, Margison GP
    • Issue date: 2001 Aug 22
    • Frequency of Ki-ras mutations and DNA alkylation in colorectal tissue from individuals living in Manchester.
    • Authors: Jackson PE, Hall CN, Badawi AF, O'Connor PJ, Cooper DP, Povey AC
    • Issue date: 1996 May
    • Development and application of a sensitive and rapid immunoassay for the quantitation of N7-methyldeoxyguanosine in DNA samples.
    • Authors: Harrison KL, Wood M, Lees NP, Hall CN, Margison GP, Povey AC
    • Issue date: 2001 Mar
    • Levels of the DNA adduct, N7-methyldeoxyguanosine, are associated with increased risk of failure of treatment of cervical intraepithelial neoplasia.
    • Authors: Acladious NN, Harrison KL, Sutton CJ, Povey AC, Mandal D, Kitchener H
    • Issue date: 2004 Jun
    • N7-methyldeoxyguanosine levels in DNA isolated from cervical cytology samples are associated with smoking.
    • Authors: Harrison KL, Khan NS, Dey P, Povey AC
    • Issue date: 2006 Aug 15
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.