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    Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays.

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    Authors
    Rhodes, Anthony
    Jasani, Bharat
    Balaton, Andre J
    Barnes, Diana M
    Anderson, Elizabeth
    Bobrow, Lynda G
    Miller, Keith D
    Affiliation
    United Kingdom National External Quality Assessment Scheme for Immunocytochemistry and the Department of Histopathology, University College London Medical School, London, England.
    Issue Date
    2001-01
    
    Metadata
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    Abstract
    Immunohistochemical assays for estrogen receptors (ERs) and progesterone receptors (PRs) have not been surveyed for technical validity. In the present study, the reliability of the immunohistochemical assay for ER and PR was evaluated using data from 105 laboratories participating in external quality assessment (EQA) during a 2-year period. Technical variables associated with reliable immunostaining were analyzed. The efficiency of the antigen retrieval step was identified as the single most important contributory factor influencing the overall reproducibility of the assays. Reliable assays were found in 24 (36%) of 66 laboratories participating in continual EQA, including the majority of centers known to have clinically validated results. Inadequate assay sensitivity, with subsequent weak staining, was the main cause of poor and variable results by laboratories using microwave antigen retrieval; too short a heating time was identified as the principal contributory factor. Extension of the heating time resulted in significant improvement regardless of all other variables in the immunohistochemical protocol. Continual participation in EQA is an effective means for identifying and ameliorating variables that influence the reliability of immunohistochemical assays for predictive markers, thereby assisting in technical validation and standardization.
    Citation
    Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays. 2001, 115 (1):44-58 Am. J. Clin. Pathol.
    Journal
    American Journal of Clinical Pathology
    URI
    http://hdl.handle.net/10541/85765
    DOI
    10.1309/H905-HYC1-6UQQ-981P
    PubMed ID
    11190807
    Type
    Article
    Language
    en
    ISSN
    0002-9173
    ae974a485f413a2113503eed53cd6c53
    10.1309/H905-HYC1-6UQQ-981P
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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