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dc.contributor.authorThomson, P J
dc.contributor.authorPotten, Christopher S
dc.contributor.authorAppleton, D R
dc.date.accessioned2009-11-10T10:38:55Z
dc.date.available2009-11-10T10:38:55Z
dc.date.issued2001-12
dc.identifier.citationIn vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity. 2001, 46 (12):1157-64 Arch. Oral Biol.en
dc.identifier.issn0003-9969
dc.identifier.pmid11684035
dc.identifier.urihttp://hdl.handle.net/10541/85764
dc.description.abstractSamples (110) of human mandibular gingiva and buccal mucosa, harvested from patients undergoing third-molar surgery, were subjected to in vitro labelling with tritiated thymidine, bromodeoxyuridine, or a sequential double-labelling technique comprising tritiated thymidine followed by bromodeoxyuridine, in order to determine the efficacy of a new incubation labelling technique, and to characterize S-phase labelling indices in human oral mucosa. Whilst, there were no demonstrable differences in labelling indices obtained by single thymidine, single bromodeoxyuridine or double labelling, there was a significant difference between anatomical sites, with higher S phase labelling observed in buccal mucosa (mean LI 11.7) than mandibular gingiva (mean LI 8.5; P<0.01). There was, however, no significant correlation between individual labelling indices and patient age, sex or the time of day when tissue was harvested. The in vitro labelling technique provides a reliable and quantifiable method of characterizing proliferative labelling indices in the human oral cavity. Further investigation is being carried out to profile wider age and anatomical ranges and to utilize the double-labelling technique to calculate S-phase durations and cell-cycle times. These profiles may have a future role in the assessment of oral mucous membrane disease.
dc.language.isoenen
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshAge Factors
dc.subject.meshBromodeoxyuridine
dc.subject.meshCell Division
dc.subject.meshCells, Cultured
dc.subject.meshCircadian Rhythm
dc.subject.meshDNA Replication
dc.subject.meshFemale
dc.subject.meshGingiva
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshMitotic Index
dc.subject.meshMouth Mucosa
dc.subject.meshS Phase
dc.subject.meshSex Factors
dc.subject.meshThymidine
dc.titleIn vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity.en
dc.typeArticleen
dc.contributor.departmentDepartment of Oral and Maxillofacial Surgery, The Dental School, University of Newcastle, Framlington Place, NE2 4BW, Newcastle upon Tyne, UK. peter.thomson@nd.ac.uken
dc.identifier.journalArchives of Oral Biologyen
html.description.abstractSamples (110) of human mandibular gingiva and buccal mucosa, harvested from patients undergoing third-molar surgery, were subjected to in vitro labelling with tritiated thymidine, bromodeoxyuridine, or a sequential double-labelling technique comprising tritiated thymidine followed by bromodeoxyuridine, in order to determine the efficacy of a new incubation labelling technique, and to characterize S-phase labelling indices in human oral mucosa. Whilst, there were no demonstrable differences in labelling indices obtained by single thymidine, single bromodeoxyuridine or double labelling, there was a significant difference between anatomical sites, with higher S phase labelling observed in buccal mucosa (mean LI 11.7) than mandibular gingiva (mean LI 8.5; P<0.01). There was, however, no significant correlation between individual labelling indices and patient age, sex or the time of day when tissue was harvested. The in vitro labelling technique provides a reliable and quantifiable method of characterizing proliferative labelling indices in the human oral cavity. Further investigation is being carried out to profile wider age and anatomical ranges and to utilize the double-labelling technique to calculate S-phase durations and cell-cycle times. These profiles may have a future role in the assessment of oral mucous membrane disease.


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