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Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.

dc.contributor.authorSlamon, N Debbie
dc.contributor.authorWard, Timothy H
dc.contributor.authorButler, John
dc.contributor.authorPentreath, Vic W
dc.date.accessioned2009-11-10T10:32:39Z
dc.date.available2009-11-10T10:32:39Z
dc.date.issued2001-06
dc.identifier.citationAssessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay. 2001, 75 (4):243-50 Arch. Toxicol.en
dc.identifier.issn0340-5761
dc.identifier.pmid11482523
dc.identifier.doi10.1007/s002040100228
dc.identifier.urihttp://hdl.handle.net/10541/85762
dc.description.abstractThe effects of acute (24 h) exposure to the antidepressants amitriptyline, imipramine (both tricyclics), fluoxetine (a selective serotonin re-uptake inhibitor) and tranylcypromine (a monoamine oxidase inhibitor) on DNA damage in cultured C6 rat glioma cells were determined using an alkaline comet assay. The effects of manipulation of intracellular cyclic AMP by pretreatment with dibutyryl cyclic AMP (dBcAMP) and 3-isobutyl-1-methylxanthine (IBMX) were also studied. For fluoxetine, the effects of addition of exogenous glutathione (GSH) and pretreatment with L-buthionine sulfoximine (BSO) were also assessed. There were increases in DNA damage with increasing concentrations of antidepressants. IBMX pretreatment protected against antidepressant-induced DNA damage in C6 cells pretreated with dBcAMP. Addition of exogenous reduced GSH and BSO increased DNA damage after fluoxetine exposure. The data show that the antidepressants induce significant amounts DNA damage in C6 cells.
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.mesh1-Methyl-3-isobutylxanthine
dc.subject.meshAnimals
dc.subject.meshAntidepressive Agents, Second-Generation
dc.subject.meshAntidepressive Agents, Tricyclic
dc.subject.meshBucladesine
dc.subject.meshButhionine Sulfoximine
dc.subject.meshComet Assay
dc.subject.meshDNA Damage
dc.subject.meshEnzyme Inhibitors
dc.subject.meshGlioma
dc.subject.meshGlutathione
dc.subject.meshPhosphodiesterase Inhibitors
dc.subject.meshRats
dc.subject.meshTumor Cells, Cultured
dc.titleAssessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biological Sciences, University of Salford, UK.en
dc.identifier.journalArchives of Toxicologyen
html.description.abstractThe effects of acute (24 h) exposure to the antidepressants amitriptyline, imipramine (both tricyclics), fluoxetine (a selective serotonin re-uptake inhibitor) and tranylcypromine (a monoamine oxidase inhibitor) on DNA damage in cultured C6 rat glioma cells were determined using an alkaline comet assay. The effects of manipulation of intracellular cyclic AMP by pretreatment with dibutyryl cyclic AMP (dBcAMP) and 3-isobutyl-1-methylxanthine (IBMX) were also studied. For fluoxetine, the effects of addition of exogenous glutathione (GSH) and pretreatment with L-buthionine sulfoximine (BSO) were also assessed. There were increases in DNA damage with increasing concentrations of antidepressants. IBMX pretreatment protected against antidepressant-induced DNA damage in C6 cells pretreated with dBcAMP. Addition of exogenous reduced GSH and BSO increased DNA damage after fluoxetine exposure. The data show that the antidepressants induce significant amounts DNA damage in C6 cells.


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