• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Slamon, N Debbie
    Ward, Timothy H
    Butler, John
    Pentreath, Vic W
    Affiliation
    Department of Biological Sciences, University of Salford, UK.
    Issue Date
    2001-06
    
    Metadata
    Show full item record
    Abstract
    The effects of acute (24 h) exposure to the antidepressants amitriptyline, imipramine (both tricyclics), fluoxetine (a selective serotonin re-uptake inhibitor) and tranylcypromine (a monoamine oxidase inhibitor) on DNA damage in cultured C6 rat glioma cells were determined using an alkaline comet assay. The effects of manipulation of intracellular cyclic AMP by pretreatment with dibutyryl cyclic AMP (dBcAMP) and 3-isobutyl-1-methylxanthine (IBMX) were also studied. For fluoxetine, the effects of addition of exogenous glutathione (GSH) and pretreatment with L-buthionine sulfoximine (BSO) were also assessed. There were increases in DNA damage with increasing concentrations of antidepressants. IBMX pretreatment protected against antidepressant-induced DNA damage in C6 cells pretreated with dBcAMP. Addition of exogenous reduced GSH and BSO increased DNA damage after fluoxetine exposure. The data show that the antidepressants induce significant amounts DNA damage in C6 cells.
    Citation
    Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay. 2001, 75 (4):243-50 Arch. Toxicol.
    Journal
    Archives of Toxicology
    URI
    http://hdl.handle.net/10541/85762
    DOI
    10.1007/s002040100228
    PubMed ID
    11482523
    Type
    Article
    Language
    en
    ISSN
    0340-5761
    ae974a485f413a2113503eed53cd6c53
    10.1007/s002040100228
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Antioxidant defense against antidepressants in C6 and 1321N1 cells.
    • Authors: Slamon ND, Pentreath VW
    • Issue date: 2000 Jul 14
    • Extending prematuration with cAMP modulators enhances the cumulus contribution to oocyte antioxidant defence and oocyte quality via gap junctions.
    • Authors: Li HJ, Sutton-McDowall ML, Wang X, Sugimura S, Thompson JG, Gilchrist RB
    • Issue date: 2016 Apr
    • Influence of glutathione levels on radiation-induced chromosomal DNA damage and repair in human peripheral lymphocytes.
    • Authors: Pujari G, Berni A, Palitti F, Chatterjee A
    • Issue date: 2009 Apr 30
    • Forskolin-stimulated cyclic AMP accumulation mediates protein synthesis-dependent refractoriness in C6-2B rat glioma cells.
    • Authors: Barovsky K, Pedone C, Brooker G
    • Issue date: 1983
    • Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro.
    • Authors: Appeltant R, Beek J, Vandenberghe L, Maes D, Van Soom A
    • Issue date: 2015 Feb
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.