Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.
Affiliation
Department of Biological Sciences, University of Salford, UK.Issue Date
2001-06
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Show full item recordAbstract
The effects of acute (24 h) exposure to the antidepressants amitriptyline, imipramine (both tricyclics), fluoxetine (a selective serotonin re-uptake inhibitor) and tranylcypromine (a monoamine oxidase inhibitor) on DNA damage in cultured C6 rat glioma cells were determined using an alkaline comet assay. The effects of manipulation of intracellular cyclic AMP by pretreatment with dibutyryl cyclic AMP (dBcAMP) and 3-isobutyl-1-methylxanthine (IBMX) were also studied. For fluoxetine, the effects of addition of exogenous glutathione (GSH) and pretreatment with L-buthionine sulfoximine (BSO) were also assessed. There were increases in DNA damage with increasing concentrations of antidepressants. IBMX pretreatment protected against antidepressant-induced DNA damage in C6 cells pretreated with dBcAMP. Addition of exogenous reduced GSH and BSO increased DNA damage after fluoxetine exposure. The data show that the antidepressants induce significant amounts DNA damage in C6 cells.Citation
Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay. 2001, 75 (4):243-50 Arch. Toxicol.Journal
Archives of ToxicologyDOI
10.1007/s002040100228PubMed ID
11482523Type
ArticleLanguage
enISSN
0340-5761ae974a485f413a2113503eed53cd6c53
10.1007/s002040100228
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