Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines.
dc.contributor.author | Sheridan, Mary T | |
dc.contributor.author | West, Catharine M L | |
dc.date.accessioned | 2009-11-10T10:27:53Z | |
dc.date.available | 2009-11-10T10:27:53Z | |
dc.date.issued | 2001-06-01 | |
dc.identifier.citation | Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines. 2001, 50 (2):503-9 Int. J. Radiat. Oncol. Biol. Phys. | en |
dc.identifier.issn | 0360-3016 | |
dc.identifier.pmid | 11380240 | |
dc.identifier.uri | http://hdl.handle.net/10541/85760 | |
dc.description.abstract | PURPOSE: To investigate the relationship between radiation-induced apoptosis and clonogenic cell kill in 9 cervical cancer cell lines. METHODS AND MATERIALS: Cells were irradiated with 0, 2, 8, and 30 Gy. The level of apoptosis was evaluated using flow cytometry (Annexin-V binding), light microscropy (morphology), gel electrophoresis (DNA ladder formation), and TUNEL assay. Cell survival was measured using a clonogenic assay. RESULTS: Of the 9 cervical carcinoma cell lines analyzed, 3 underwent radiation-induced apoptosis: CaSki, HT3, and 778. The levels of apoptosis, obtained 72 h after a dose of 30 Gy, were 49%, 28%, and 26%, respectively. All cell lines exhibited some level of background apoptosis measured by Annexin-V binding (mean = 2.6%+/-0.8; range, 0.2-6.9%) that correlated with the level of radiation-induced apoptosis (r = 0.92, p = 0.001). In 6 of the 9 lines, necrosis was the dominant form of cell death. A significant inverse relationship was found between the level of radiation-induced apoptosis and necrosis after 30 Gy (r = -0.87, p = 0.002). No relationship was found between radiation-induced apoptosis and intrinsic radiosensitivity measured, using a clonogenic assay, as surviving fraction at 2 Gy (SF2). CONCLUSION: Cervical carcinoma cells do not readily undergo radiation-induced apoptosis in vitro. There is no relationship between ability to undergo apoptosis and intrinsic radiosensitivity measured using a clonogenic assay. | |
dc.language.iso | en | en |
dc.subject | Cultured Tumour Cells | en |
dc.subject | Uterine Cervical Cancer | en |
dc.subject.mesh | Apoptosis | |
dc.subject.mesh | Cell Death | |
dc.subject.mesh | Female | |
dc.subject.mesh | Hela Cells | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Necrosis | |
dc.subject.mesh | Radiation Tolerance | |
dc.subject.mesh | Tumor Cells, Cultured | |
dc.subject.mesh | Uterine Cervical Neoplasms | |
dc.title | Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines. | en |
dc.type | Article | en |
dc.contributor.department | CRC Experimental Radiation Oncology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom. | en |
dc.identifier.journal | International Journal of Radiation Oncology, Biology, Physics | en |
html.description.abstract | PURPOSE: To investigate the relationship between radiation-induced apoptosis and clonogenic cell kill in 9 cervical cancer cell lines. METHODS AND MATERIALS: Cells were irradiated with 0, 2, 8, and 30 Gy. The level of apoptosis was evaluated using flow cytometry (Annexin-V binding), light microscropy (morphology), gel electrophoresis (DNA ladder formation), and TUNEL assay. Cell survival was measured using a clonogenic assay. RESULTS: Of the 9 cervical carcinoma cell lines analyzed, 3 underwent radiation-induced apoptosis: CaSki, HT3, and 778. The levels of apoptosis, obtained 72 h after a dose of 30 Gy, were 49%, 28%, and 26%, respectively. All cell lines exhibited some level of background apoptosis measured by Annexin-V binding (mean = 2.6%+/-0.8; range, 0.2-6.9%) that correlated with the level of radiation-induced apoptosis (r = 0.92, p = 0.001). In 6 of the 9 lines, necrosis was the dominant form of cell death. A significant inverse relationship was found between the level of radiation-induced apoptosis and necrosis after 30 Gy (r = -0.87, p = 0.002). No relationship was found between radiation-induced apoptosis and intrinsic radiosensitivity measured, using a clonogenic assay, as surviving fraction at 2 Gy (SF2). CONCLUSION: Cervical carcinoma cells do not readily undergo radiation-induced apoptosis in vitro. There is no relationship between ability to undergo apoptosis and intrinsic radiosensitivity measured using a clonogenic assay. |