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    Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1.

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    Authors
    Ray, K
    Embleton, Jim
    Jailkhani, B L
    Bhan, M K
    Kumar, R
    Affiliation
    Department of Paediatrics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
    Issue Date
    2001-07
    
    Metadata
    Show full item record
    Abstract
    We have prepared human recombinant antibody molecules against the glycoprotein antigen of the rabies virus (GPRV) based on the single chain variable fragment (scFv) format. Anti-GPRV scFvs were selected from a human synthetic scFv phage display library with a repertoire of approximately 109 specificities. After three rounds of selection against the PV11 strain of the virus, 40% of the clones tested recognized the rabies antigen. Of the 20 positive clones that were sequenced, five distinct sequences were identified. These distinct scFvs were cloned into a mammalian expression vector carrying the human IgG1 Fc region. The specificity of the resulting scFv-Fc molecules for GPRV was established by ELISA, dot blot and western blot analyses and membrane immunofluorescence. Two of the scFv-Fc fusion proteins neutralized the PV11 strain in a standard in vivo neutralization assay where the virus was incubated with the scFv-Fc molecules before intracranial inoculation in mice. These anti-GPRV scFv-Fc molecules have the potential to be used as an alternative to the presently available HRIG, for use in post-exposure preventive treatment.
    Citation
    Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1. 2001, 125 (1):94-101 Clin. Exp. Immunol.
    Journal
    Clinical and Experimental Immunology
    URI
    http://hdl.handle.net/10541/85755
    PubMed ID
    11472431
    Type
    Article
    Language
    en
    ISSN
    0009-9104
    Collections
    All Paterson Institute for Cancer Research

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