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dc.contributor.authorMasters, J R
dc.contributor.authorThomson, J A
dc.contributor.authorDaly-Burns, B
dc.contributor.authorReid, Y A
dc.contributor.authorDirks, W G
dc.contributor.authorPacker, P
dc.contributor.authorToji, L H
dc.contributor.authorOhno, T
dc.contributor.authorTanabe, H
dc.contributor.authorArlett, C F
dc.contributor.authorKelland, L R
dc.contributor.authorHarrison, M
dc.contributor.authorVirmani, A
dc.contributor.authorWard, Timothy H
dc.contributor.authorAyres, K L
dc.contributor.authorDebenham, P G
dc.date.accessioned2009-11-10T09:59:53Z
dc.date.available2009-11-10T09:59:53Z
dc.date.issued2001-07-03
dc.identifier.citationShort tandem repeat profiling provides an international reference standard for human cell lines. 2001, 98 (14):8012-7 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn0027-8424
dc.identifier.pmid11416159
dc.identifier.doi10.1073/pnas.121616198
dc.identifier.urihttp://hdl.handle.net/10541/85729
dc.description.abstractCross-contamination between cell lines is a longstanding and frequent cause of scientific misrepresentation. Estimates from national testing services indicate that up to 36% of cell lines are of a different origin or species to that claimed. To test a standard method of cell line authentication, 253 human cell lines from banks and research institutes worldwide were analyzed by short tandem repeat profiling. The short tandem repeat profile is a simple numerical code that is reproducible between laboratories, is inexpensive, and can provide an international reference standard for every cell line. If DNA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated.
dc.language.isoenen
dc.subject.meshCell Line
dc.subject.meshGene Expression Profiling
dc.subject.meshHumans
dc.subject.meshReference Standards
dc.subject.meshTandem Repeat Sequences
dc.titleShort tandem repeat profiling provides an international reference standard for human cell lines.en
dc.typeArticleen
dc.contributor.departmentInstitute of Urology, University College London, 3rd Floor Research Laboratories, 67 Riding House Street, London W1W 7EY, United Kingdom. J.Masters@Ucl.ac.uken
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen
html.description.abstractCross-contamination between cell lines is a longstanding and frequent cause of scientific misrepresentation. Estimates from national testing services indicate that up to 36% of cell lines are of a different origin or species to that claimed. To test a standard method of cell line authentication, 253 human cell lines from banks and research institutes worldwide were analyzed by short tandem repeat profiling. The short tandem repeat profile is a simple numerical code that is reproducible between laboratories, is inexpensive, and can provide an international reference standard for every cell line. If DNA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated.


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