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dc.contributor.authorMarshman, Emma
dc.contributor.authorOttewell, Penny
dc.contributor.authorPotten, Christopher S
dc.contributor.authorWatson, Alastair
dc.date.accessioned2009-11-10T09:59:00Z
dc.date.available2009-11-10T09:59:00Z
dc.date.issued2001-10
dc.identifier.citationCaspase activation during spontaneous and radiation-induced apoptosis in the murine intestine. 2001, 195 (3):285-92 J. Pathol.en
dc.identifier.issn0022-3417
dc.identifier.pmid11673824
dc.identifier.doi10.1002/path.967
dc.identifier.urihttp://hdl.handle.net/10541/85728
dc.description.abstractThe aim of this study was to characterize the activation of caspase-3 along the crypt/villus axis in the normal and irradiated intestine and to compare active caspase-3 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8 Gy gamma-radiation. Positive apoptotic cells stained with an antibody against active caspase-3, haematoxylin and eosin (H&E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase-3 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase-3 expression gave similar results to apoptosis detected from H&E-stained sections. In the normal villus, active caspase-3 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&E sections. This study indicates that caspase-3 is activated in intestinal crypts but not in villi following gamma-radiation. Active caspase-3 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshApoptosis
dc.subject.meshCaspase 3
dc.subject.meshCaspases
dc.subject.meshColon
dc.subject.meshEnzyme Activation
dc.subject.meshEosine Yellowish-(YS)
dc.subject.meshEpithelium
dc.subject.meshHematoxylin
dc.subject.meshImmunohistochemistry
dc.subject.meshIn Situ Nick-End Labeling
dc.subject.meshIntestine, Small
dc.subject.meshIntestines
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred Strains
dc.subject.meshStaining and Labeling
dc.titleCaspase activation during spontaneous and radiation-induced apoptosis in the murine intestine.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, University of Liverpool, UCD Duncan Building, Daulby Street, Liverpool, L69 3GA, UK.en
dc.identifier.journalThe Journal of Pathologyen
html.description.abstractThe aim of this study was to characterize the activation of caspase-3 along the crypt/villus axis in the normal and irradiated intestine and to compare active caspase-3 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8 Gy gamma-radiation. Positive apoptotic cells stained with an antibody against active caspase-3, haematoxylin and eosin (H&E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase-3 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase-3 expression gave similar results to apoptosis detected from H&E-stained sections. In the normal villus, active caspase-3 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&E sections. This study indicates that caspase-3 is activated in intestinal crypts but not in villi following gamma-radiation. Active caspase-3 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification.


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