• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Marshman, Emma
    Ottewell, Penny
    Potten, Christopher S
    Watson, Alastair
    Affiliation
    Department of Medicine, University of Liverpool, UCD Duncan Building, Daulby Street, Liverpool, L69 3GA, UK.
    Issue Date
    2001-10
    
    Metadata
    Show full item record
    Abstract
    The aim of this study was to characterize the activation of caspase-3 along the crypt/villus axis in the normal and irradiated intestine and to compare active caspase-3 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8 Gy gamma-radiation. Positive apoptotic cells stained with an antibody against active caspase-3, haematoxylin and eosin (H&E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase-3 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase-3 expression gave similar results to apoptosis detected from H&E-stained sections. In the normal villus, active caspase-3 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&E sections. This study indicates that caspase-3 is activated in intestinal crypts but not in villi following gamma-radiation. Active caspase-3 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification.
    Citation
    Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine. 2001, 195 (3):285-92 J. Pathol.
    Journal
    The Journal of Pathology
    URI
    http://hdl.handle.net/10541/85728
    DOI
    10.1002/path.967
    PubMed ID
    11673824
    Type
    Article
    Language
    en
    ISSN
    0022-3417
    ae974a485f413a2113503eed53cd6c53
    10.1002/path.967
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Burn-induced gut mucosal homeostasis in TCR delta receptor-deficient mice.
    • Authors: Wu X, Woodside KJ, Song J, Wolf SE
    • Issue date: 2004 Jan
    • Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts.
    • Authors: Duan WR, Garner DS, Williams SD, Funckes-Shippy CL, Spath IS, Blomme EA
    • Issue date: 2003 Feb
    • General suitability of techniques for in situ detection of apoptosis in small intestinal epithelium.
    • Authors: Groos S, Reale E, Luciano L
    • Issue date: 2003 Jun
    • Induction of apoptosis before shedding of human intestinal epithelial cells.
    • Authors: Grossmann J, Walther K, Artinger M, Rümmele P, Woenckhaus M, Schölmerich J
    • Issue date: 2002 Jun
    • Mechanisms underlying the radioprotective effect of histamine on small intestine.
    • Authors: Medina VA, Croci M, Mohamad NA, Massari N, Garbarino G, Cricco GP, Núñez MA, Martín GA, Crescenti EJ, Bergoc RM, Rivera ES
    • Issue date: 2007 Oct
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.